Shoorvir V. Singh, A. Singh, P. Singh, Saurabh Gupta, H. Singh, Brajesh K. Singh, O. R. V. Kumar, A. S. Rajendiran, N. Swain, J. Sohal
{"title":"利用山羊源禽分枝杆菌亚种副结核毒株“S5”的“印度野牛型”基因型在一群羊约翰氏病地方性流行中开发的“本土疫苗”的评价:在印度进行的三年试验","authors":"Shoorvir V. Singh, A. Singh, P. Singh, Saurabh Gupta, H. Singh, Brajesh K. Singh, O. R. V. Kumar, A. S. Rajendiran, N. Swain, J. Sohal","doi":"10.4236/WJV.2013.32009","DOIUrl":null,"url":null,"abstract":"“Indigenous vaccine” developed from native “Indian Bison Type” strain (“S5”) \nof Mycobacterium avium subspecies paratuberculosis (MAP) of “goat origin” was first time evaluated in a sheep \nflock of Bharat Merino breed located in Mannavanur town of Tamil Nadu in South \nIndia. Therapeutic efficacy of the vaccine was evaluated for 3 years between \n2008 and 2010, on the basis of improvements in productivity (body weights, \nreproductive efficiency and survivability), physical condition, clinical \nsymptoms (weakness, diarrhea, wool quality), immune response (sero-conversion) \nand infection load in feces (shedding). After immunization of the flock in 2008, \nthe successive progenies of 112 and 53 lambs born in 2009 and 2010, \nrespectively were vaccinated. Whereas, 40 lambs born to control animals were \nkept as unvaccinated controls. Though gain in body weights in vaccinated versus \ncontrols were not significant in 2008, growth rates were distinctly superior in \nIst and IInd generations of vaccinated lambs. \nReproductive performance (tupping percent) and survivability of lambs and adult \nsheep improved significantly. There was \noverall reduction in yearly morbidity (diarrhea) and mortality rates of \nthe flock in post vaccination years. Shedding of MAP in feces was reduced in \nvaccinated sheep by 6.2%, 14.3% and 27.3% in 2008, 2009 and 2010 respectively, whereas shedding increased in the \ncontrol sheep. Seromonitoring \nof the animals by “indigenous \nELISA kit” showed enhanced “flock immunity” in successive generations. “Indigenous vaccine” reduced clinical disease and shedding \nand improved immunity and productivity of Bharat Merino flock, endemic for \nJohne’s disease.","PeriodicalId":57190,"journal":{"name":"疫苗(英文)","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2013-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"13","resultStr":"{\"title\":\"Evaluation of “Indigenous Vaccine” Developed Using “Indian Bison Type” Genotype of Mycobacterium avium subspecies paratuberculosis Strain “S5” of Goat Origin in a Sheep Flock Endemic for Johne’s Disease: A Three Years Trial in India\",\"authors\":\"Shoorvir V. Singh, A. Singh, P. Singh, Saurabh Gupta, H. Singh, Brajesh K. Singh, O. R. V. Kumar, A. S. Rajendiran, N. Swain, J. Sohal\",\"doi\":\"10.4236/WJV.2013.32009\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"“Indigenous vaccine” developed from native “Indian Bison Type” strain (“S5”) \\nof Mycobacterium avium subspecies paratuberculosis (MAP) of “goat origin” was first time evaluated in a sheep \\nflock of Bharat Merino breed located in Mannavanur town of Tamil Nadu in South \\nIndia. Therapeutic efficacy of the vaccine was evaluated for 3 years between \\n2008 and 2010, on the basis of improvements in productivity (body weights, \\nreproductive efficiency and survivability), physical condition, clinical \\nsymptoms (weakness, diarrhea, wool quality), immune response (sero-conversion) \\nand infection load in feces (shedding). After immunization of the flock in 2008, \\nthe successive progenies of 112 and 53 lambs born in 2009 and 2010, \\nrespectively were vaccinated. Whereas, 40 lambs born to control animals were \\nkept as unvaccinated controls. Though gain in body weights in vaccinated versus \\ncontrols were not significant in 2008, growth rates were distinctly superior in \\nIst and IInd generations of vaccinated lambs. \\nReproductive performance (tupping percent) and survivability of lambs and adult \\nsheep improved significantly. There was \\noverall reduction in yearly morbidity (diarrhea) and mortality rates of \\nthe flock in post vaccination years. Shedding of MAP in feces was reduced in \\nvaccinated sheep by 6.2%, 14.3% and 27.3% in 2008, 2009 and 2010 respectively, whereas shedding increased in the \\ncontrol sheep. Seromonitoring \\nof the animals by “indigenous \\nELISA kit” showed enhanced “flock immunity” in successive generations. “Indigenous vaccine” reduced clinical disease and shedding \\nand improved immunity and productivity of Bharat Merino flock, endemic for \\nJohne’s disease.\",\"PeriodicalId\":57190,\"journal\":{\"name\":\"疫苗(英文)\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-05-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"13\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"疫苗(英文)\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.4236/WJV.2013.32009\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"疫苗(英文)","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.4236/WJV.2013.32009","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Evaluation of “Indigenous Vaccine” Developed Using “Indian Bison Type” Genotype of Mycobacterium avium subspecies paratuberculosis Strain “S5” of Goat Origin in a Sheep Flock Endemic for Johne’s Disease: A Three Years Trial in India
“Indigenous vaccine” developed from native “Indian Bison Type” strain (“S5”)
of Mycobacterium avium subspecies paratuberculosis (MAP) of “goat origin” was first time evaluated in a sheep
flock of Bharat Merino breed located in Mannavanur town of Tamil Nadu in South
India. Therapeutic efficacy of the vaccine was evaluated for 3 years between
2008 and 2010, on the basis of improvements in productivity (body weights,
reproductive efficiency and survivability), physical condition, clinical
symptoms (weakness, diarrhea, wool quality), immune response (sero-conversion)
and infection load in feces (shedding). After immunization of the flock in 2008,
the successive progenies of 112 and 53 lambs born in 2009 and 2010,
respectively were vaccinated. Whereas, 40 lambs born to control animals were
kept as unvaccinated controls. Though gain in body weights in vaccinated versus
controls were not significant in 2008, growth rates were distinctly superior in
Ist and IInd generations of vaccinated lambs.
Reproductive performance (tupping percent) and survivability of lambs and adult
sheep improved significantly. There was
overall reduction in yearly morbidity (diarrhea) and mortality rates of
the flock in post vaccination years. Shedding of MAP in feces was reduced in
vaccinated sheep by 6.2%, 14.3% and 27.3% in 2008, 2009 and 2010 respectively, whereas shedding increased in the
control sheep. Seromonitoring
of the animals by “indigenous
ELISA kit” showed enhanced “flock immunity” in successive generations. “Indigenous vaccine” reduced clinical disease and shedding
and improved immunity and productivity of Bharat Merino flock, endemic for
Johne’s disease.