Sunanda Burman, Shubhaisi Das, B. Singh, S. Chakraborty, N. Sultana, Rimpa Ghosh, A. Mandal, Bipul Ghosh, G. Chandra
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Highest mortality (100%) was recorded at 100 ppm concentration of methanol extract after 72 hours in 1, 2 and 3 instars larvae. LC50 and LC90 values were 72.32 and 131.14 ppm respectively after 24 h for the 3 instar larvae. No effects on non-target organisms found. Crude and methanol extracts showed antibacterial activity against all tested bacteria while aqueous extracts showed none. Highest inhibition zone (19.00±0.00 mm) was observed against Pseudomonas fluorescens MTCC 103 by methanolic extract. Preliminary phytochemical screening of most potent extract detected tannins, flavonoids, alkaloids and terpenoids. Presence of various functional groups was revealed by FT-IR analysis. Results confirm the potential mosquito larvicidal and antibacterial activities of mixture C. amada rhizome and T. indica leaf extracts. 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引用次数: 2
摘要
本研究旨在评价姜黄根与柽柳叶的粗提物与溶剂提物的混合物对vishnui库蚊幼虫和8种致病菌的杀蚊抑菌活性。采用不同浓度的粗提物、甲醇和水提物进行杀幼虫生物测定。对LC50和LC90值进行Log-probit分析。并进行方差分析。此外,还研究了提取物对非靶生物的影响。采用标准方法测定其抑菌活性。对最有效的提取物进行了初步的植物化学分析和傅里叶变换红外光谱分析。1、2、3龄幼虫在浓度为100 ppm的甲醇提取物处理72 h后死亡率最高(100%)。3龄幼虫24 h LC50和LC90值分别为72.32和131.14 ppm。对非目标生物无影响。粗提物和甲醇提物对所有细菌均有抑菌活性,而水提物无抑菌活性。甲醇提取物对荧光假单胞菌MTCC 103的最大抑制区为19.00±0.00 mm。初步植物化学筛选最有效的提取物检测到单宁、黄酮类、生物碱和萜类。FT-IR分析显示了各种官能团的存在。结果表明,野田菊和野田菊叶提取物的混合提取物具有潜在的杀蚊和抑菌活性。这种利用混合萃取物对抗有害生物制剂的方法是一种很有前景的发现,它可能进一步批准植物源性生物活性化合物协同作用的应用。
Assessment of Mosquitocidal and Antibacterial Activities of Mixture of Different Extracts of Curcuma amada Rhizome and Tamarindus indica Leaves
Present study aimed at evaluation of mosquito larvicidal and antibacterial activities of mixture of crude and solvent extracts of Curcuma amada rhizome and Tamarindus indica leaf against Culex vishnui larvae and eight pathogenic bacteria. Larvicidal bioassay was performed by graded concentrations of crude, methanol and aqueous extracts. Log-probit analysis for estimation of LC50 and LC90 values was performed. ANOVA analysis was also conducted. Effects of the extracts on non-target organisms were also examined. Antibacterial activity was examined through standard methods. Preliminary phytochemical analysis and Fourier Transform Infra Red (FT-IR) spectroscopic analysis of the most potent extract were also done. Highest mortality (100%) was recorded at 100 ppm concentration of methanol extract after 72 hours in 1, 2 and 3 instars larvae. LC50 and LC90 values were 72.32 and 131.14 ppm respectively after 24 h for the 3 instar larvae. No effects on non-target organisms found. Crude and methanol extracts showed antibacterial activity against all tested bacteria while aqueous extracts showed none. Highest inhibition zone (19.00±0.00 mm) was observed against Pseudomonas fluorescens MTCC 103 by methanolic extract. Preliminary phytochemical screening of most potent extract detected tannins, flavonoids, alkaloids and terpenoids. Presence of various functional groups was revealed by FT-IR analysis. Results confirm the potential mosquito larvicidal and antibacterial activities of mixture C. amada rhizome and T. indica leaf extracts. This approach of using mixture of extracts for getting better result against harmful biological agents is a promising finding which may further approve application of coactivity of bioactive compounds of plant origin.