甘草酸、甜菊苷和三氯蔗糖对普遍存在的肠道共生大肠杆菌生长和代谢的影响

Q3 Biochemistry, Genetics and Molecular Biology
Samayeta Tuli, Saadlee Shehreen, Abira Khan, S. Akhteruzzaman, A. Sajib
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引用次数: 0

摘要

无热量甜味剂(NCS)在肥胖和糖尿病患者中越来越受欢迎,因为它们能够在不输入任何热量的情况下提供甜味。然而,食用NCS可能与代谢紊乱有关。这些不良反应背后的机制尚不清楚,这表明NCS可能改变肠道微生物群的代谢活性,导致肠道环境扰动,导致宿主生理异常。这项研究调查了大肠杆菌(一种常见的肠道微生物)对三种NCS的反应,其中两种是天然的(甘草酸苷和甜菊糖苷),另一种是人工合成的(三氯蔗糖)。为了评估这些NCS的影响,本研究调查了大肠杆菌在不同甜味剂负荷下的生长、基因表达和代谢途径。与未经处理的对照组相比,少量的甘草酸使大肠杆菌生长得更快,而等量的三氯蔗糖和甜菊糖甙没有或不太明显的影响。在较高浓度下,这些甜味剂减缓或抑制了细菌的生长。因此,NCS可能对肠道微生物有剂量依赖性影响。在与每日可接受摄入量相对应的剂量治疗后,关键代谢基因的表达出现改变,这可能会影响它们的代谢。在不同浓度的NCS下,对照和不同NCS处理之间的总体代谢组学特征存在明显差异。在体外条件下,甘草酸和三氯蔗糖对大肠杆菌生长、基因表达和代谢的影响差异较小,这可能表明它们对人类来说是相对安全的糖替代品。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of glycyrrhizin, stevioside, and sucralose on the growth and metabolism of the omnipresent gut commensal Escherichia coli
The rise in popularity of non-caloric sweeteners (NCS) among obese and diabetic patients is due to their ability to provide a sweet taste without any caloric input. However, NCS consumption may be linked to metabolic disorders. The mechanisms behind these adverse effects are still unclear, which suggests that NCS may alter the metabolic activity of the gut microbiome, leading to enteric environmental perturbation and resulting in physiological anomalies in the host. This study investigates how Escherichia coli, a common gut microbe, reacts to three NCS, two of which come from nature (glycyrrhizin and stevioside) and one that is made artificially (sucralose). To assess the impact of these NCS, this study investigated the growth, gene expression, and metabolic pathways of E. coli under different sweetener loads. Compared to the untreated control, low amounts of glycyrrhizin made E. coli grow faster, while similar amounts of sucralose and stevioside had no or a less noticeable effect. At higher concentrations, these sweeteners slowed down or inhibited the growth of the bacteria. Thus, NCS may have dose-dependent impacts on gut microbes. After treatment with amounts that corresponded to the acceptable daily intake, the expression of key metabolic genes showed altered expression, which may potentially affect their metabolism. At different concentrations of the NCS, there were clear differences in the global metabolomic profile between the control and the different NCS treatments. Under in vitro conditions, glycyrrhizin and sucralose appeared to have a less divergent impact on E. coli growth, gene expression, and metabolism, which may suggest that these are relatively safer sugar substitutes for humans.
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来源期刊
Journal of Advanced Biotechnology and Experimental Therapeutics
Journal of Advanced Biotechnology and Experimental Therapeutics Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.90
自引率
0.00%
发文量
41
审稿时长
8 weeks
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