不同小麦草品种酚类化合物及其抗氧化活性测定的新方法

Q3 Environmental Science
Ekoloji Pub Date : 2014-03-24 DOI:10.5053/EKOLOJI.2014.909
Tuğba Akcan Kardaş, I. Durucasu
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引用次数: 14

摘要

以小麦草、土耳其琥珀硬粒小麦为研究对象,对其酚类物质含量和抗氧化能力进行了研究。为了这个目的,土耳其琥珀Durum小麦在三个不同的时间(15,30和40天)发芽。所有的小麦样品都在相同的有机条件下发芽,然后在没有阳光的情况下用空气干燥,然后放在50°C的培养箱里过夜。用乙酸乙酯提取干燥和粉碎后的琥珀麦草,比较其对2,2-二苯基-1苦酰肼(DPPH)自由基的抗氧化能力,同时测定其总酚含量(TPC)。采用高效液相色谱法测定提取物中酚类化合物含量。本研究采用新方法,采用HPLC系统在50 min内分别检测出9种标准品。在小麦草中鉴定出的主要酚类物质为阿魏酸、没食子酸、咖啡酸、香豆酸、鞣花酸、苯甲酸、对羟基苯甲酸、丁香酸、槲皮素和丁基羟基茴香醚。发芽15 d的土耳其琥珀小麦抗氧化和抗自由基活性最高。本研究表明,小麦草的重要性在于其具有较高的抗氧化活性。食用小麦草对人体健康有积极影响,如降低冠心病、某些类型的癌症和中风的风险。由于小麦草的高抗氧化作用,目的是将其用作食品防腐剂。当小麦草作为天然食品防腐剂使用时,会保留合成食品防腐剂对人体的致癌作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A New Analytical Method for the Determination of Phenolic Compounds and Their Antioxidant Activities in Different Wheat Grass Varieties
In this work, the phenolic content and antioxidant capacity of wheat grass, Turkish Amber Durum wheat was studied. For this purpose Turkish Amber Durum wheat was germinated at three different times (15, 30 and 40 days). All the wheat samples sprouted in the same organic conditions and then dried with air and without sunlight and placed into an incubator at 50°C overnight. The dried and milled amber wheat grass was extracted with ethyl acetate and compared for their antioxidant capacities against the 2,2-diphenyl-1picrylhydrazyl (DPPH.) radical, and at the same time the total phenolic content (TPC) was determined. Phenolic compounds in the extracts were examined by high performance liquid chromatography (HPLC). In this study, by using a new method, nine standards were separately detected within 50 min by using the HPLC system, as previously reported by other authors. The major phenolics, identified in the wheat grass that consisted of ferulic acid, gallic acid, caffeic acid, pcoumaric acid, ellagic acid, benzoic acid, p-hydroxybenzoic acid, syringic acid, quercetin and bha (butylated hydroxyanisole). The highest antioxidant and antiradical activities were detected in Turkish Amber wheat that sprouted in 15 days. This study demonstrates that the importance of wheat grass is due to its high antioxidant activity. Consumption of wheat grass has positive effects on human health such as reduced risk of coronary heart diseases, certain type of cancers and stroke. Due to the high antioxidant effect of wheat grass the aim is to use it as a food preservative. When wheat grass will be used as a natural food preservative, carcinogenic effects of synthetic food preservatives for body would be preserved.
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来源期刊
Ekoloji
Ekoloji 环境科学-生态学
CiteScore
1.10
自引率
0.00%
发文量
0
审稿时长
>12 weeks
期刊介绍: Cessation. Ekoloji is an international journal that focuses on papers that report results from original research on all disciplines engaged in the field of environmental research. We welcome articles that cover the entire spectrum of environmental problems and environmental pollutants, whether chemical, biological or physical. Its coverage extends to all environmentally related issues: air and water pollution, solid waste, noise, recycling, natural resources, ecology and environmental protection. It includes articles on basic and applied environmental pollution research, including environmental engineering and environmental health. All types of pollution are covered, including atmospheric pollutants, detergents, fertilizers, industrial effluents, metals, mining wastes, oil, pesticides, plastics, radioactive materials and sewage. It also includes research papers on ecological and environmental issues such as climate change, biodiversity. The primary criteria for publication are scientific quality and ecological/environmental significance. The journal will be read and contributed to by biologists, applied ecologists, environmental scientists, natural resource specialists, environmental engineers, environmental health specialists, agro-ecologists, veterinaries, agricultural engineers, landscape planners and designers. The journal welcomes full "research papers" and short "research notes", only in the English language.
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