{"title":"盐胁迫下黑穗草质膜Na7H+反转运蛋白(SOS1)基因的分离与鉴定","authors":"Banafsheh Taherinia, H. Kavousi, Sara Dehghan","doi":"10.5053/EJOBIOS.2015.9.0.2","DOIUrl":null,"url":null,"abstract":"Background: Leptochloa fusca is a halophyte plant which is highly tolerant to saline and sodic soils and water. Moreover, L fusca is an attractive model plant to study the mechanism of salt tolerance mainly due to its characteristics as a typical euhalophyte, having both accumulating and excreting salt properties. Soil salinity adversely affects plant growth, development and disturbs intracellular ion homeostasis resulting cellular toxicity. The Salt Overly Sensitive 1 (SOS1) gene encodes a plasma membrane NaVH+ antiporter that plays an important role in imparting salt stress tolerance of plants. \nMaterial and Methods: Using conserved sequences of S O S 1 , the coding sequence of plasma membrane NaVH+ antiporter (SOS1) in kallar grass was partially isolated and its expression profile during salinity stress was investigated. \nResults: The aa (amino acid) sequence of the isolated region of /./SOS1 possesses the maximum identity up to 96% of its orthologue in Distichlis spicata. The results of semi-quantitative RT-PCR revealed that salinization was affected SOS1 transcript level positively. The expression of i./50S1 in leaves of kallar grass progressively increased under all salinity levels compared to control. \nConclusions: The results suggest that i./SOS1 may play an essential role in the salt tolerance of L fusca and may be useful for improving salt tolerance in other crop species.","PeriodicalId":11848,"journal":{"name":"Eurasian Journal of Biosciences","volume":"9 1","pages":"12-20"},"PeriodicalIF":0.0000,"publicationDate":"2015-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5053/EJOBIOS.2015.9.0.2","citationCount":"5","resultStr":"{\"title\":\"Isolation and characterization of plasma membrane Na7H+ antiporter (SOS1) gene during salinity stress in kallar grass {Leptochloa fusca)\",\"authors\":\"Banafsheh Taherinia, H. Kavousi, Sara Dehghan\",\"doi\":\"10.5053/EJOBIOS.2015.9.0.2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: Leptochloa fusca is a halophyte plant which is highly tolerant to saline and sodic soils and water. Moreover, L fusca is an attractive model plant to study the mechanism of salt tolerance mainly due to its characteristics as a typical euhalophyte, having both accumulating and excreting salt properties. Soil salinity adversely affects plant growth, development and disturbs intracellular ion homeostasis resulting cellular toxicity. The Salt Overly Sensitive 1 (SOS1) gene encodes a plasma membrane NaVH+ antiporter that plays an important role in imparting salt stress tolerance of plants. \\nMaterial and Methods: Using conserved sequences of S O S 1 , the coding sequence of plasma membrane NaVH+ antiporter (SOS1) in kallar grass was partially isolated and its expression profile during salinity stress was investigated. \\nResults: The aa (amino acid) sequence of the isolated region of /./SOS1 possesses the maximum identity up to 96% of its orthologue in Distichlis spicata. The results of semi-quantitative RT-PCR revealed that salinization was affected SOS1 transcript level positively. The expression of i./50S1 in leaves of kallar grass progressively increased under all salinity levels compared to control. \\nConclusions: The results suggest that i./SOS1 may play an essential role in the salt tolerance of L fusca and may be useful for improving salt tolerance in other crop species.\",\"PeriodicalId\":11848,\"journal\":{\"name\":\"Eurasian Journal of Biosciences\",\"volume\":\"9 1\",\"pages\":\"12-20\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.5053/EJOBIOS.2015.9.0.2\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Eurasian Journal of Biosciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5053/EJOBIOS.2015.9.0.2\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Eurasian Journal of Biosciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5053/EJOBIOS.2015.9.0.2","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Isolation and characterization of plasma membrane Na7H+ antiporter (SOS1) gene during salinity stress in kallar grass {Leptochloa fusca)
Background: Leptochloa fusca is a halophyte plant which is highly tolerant to saline and sodic soils and water. Moreover, L fusca is an attractive model plant to study the mechanism of salt tolerance mainly due to its characteristics as a typical euhalophyte, having both accumulating and excreting salt properties. Soil salinity adversely affects plant growth, development and disturbs intracellular ion homeostasis resulting cellular toxicity. The Salt Overly Sensitive 1 (SOS1) gene encodes a plasma membrane NaVH+ antiporter that plays an important role in imparting salt stress tolerance of plants.
Material and Methods: Using conserved sequences of S O S 1 , the coding sequence of plasma membrane NaVH+ antiporter (SOS1) in kallar grass was partially isolated and its expression profile during salinity stress was investigated.
Results: The aa (amino acid) sequence of the isolated region of /./SOS1 possesses the maximum identity up to 96% of its orthologue in Distichlis spicata. The results of semi-quantitative RT-PCR revealed that salinization was affected SOS1 transcript level positively. The expression of i./50S1 in leaves of kallar grass progressively increased under all salinity levels compared to control.
Conclusions: The results suggest that i./SOS1 may play an essential role in the salt tolerance of L fusca and may be useful for improving salt tolerance in other crop species.
期刊介绍:
EurAsian Journal of BioSciences (Abbrev. Eurasia J Biosci or EJOBIOS) is an international, refereed electronic journal. It publishes the results of original research in the field of biological sciences restricted tomorphology, physiology, genetics, taxonomy, ecology and biogeography of both prokaryotic and eucaryotic organisms. The journal encourages submission of manuscripts dealing with plant biology, animal biology, plant physiology, microbiology, hydrobiology, ecology and environmental science, ethnobiology, biodiversity and conservation biology. EurAsian Journal of BioSciences publishes original articles in the following areas: -Agriculture, Fisheries & Food -Anatomy & Morphology -Behavioural Sciences -Biology, Biochemistry and Biotechnology -Biophysics -Biology Education -Cellular Biology and Anatomical Sciences -Ecology, Evolution & Environment -Entomology -Forestry -General Biology -Genetics & Heredity -Life Sciences - Other topics -Microbiology and Immunology -Molecular Biology -Mycology -Palaeontology -Parasitology -Pharmacology & Pharmacy -Physiology and Related Sciences -Plant Sciences -Toxicology -Veterinary Sciences -Virology -Zoology