盐胁迫下黑穗草质膜Na7H+反转运蛋白(SOS1)基因的分离与鉴定

Q3 Agricultural and Biological Sciences
Banafsheh Taherinia, H. Kavousi, Sara Dehghan
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引用次数: 5

摘要

背景:镰刀细藻是一种盐生植物,对盐碱地和水具有高度的耐受性。此外,扶桑是一种典型的真盐植物,具有积累和排泄盐的特性,是研究扶桑耐盐机理的一个有吸引力的模式植物。土壤盐分对植物生长发育有不利影响,扰乱细胞内离子平衡,导致细胞毒性。盐过度敏感1 (SOS1)基因编码一个质膜NaVH+反转运蛋白,在植物耐盐性中起重要作用。材料与方法:利用SOS1的保守序列,分离部分芥蓝质膜NaVH+反转运蛋白(SOS1)编码序列,研究其在盐胁迫下的表达谱。结果:分离区aa(氨基酸)序列。/SOS1在spicata中具有高达96%的同源性。半定量RT-PCR结果显示,盐碱化对SOS1转录物水平有正向影响。与对照相比,在不同盐度条件下,黑穗草叶片中i./50S1的表达量逐渐增加。结论:i./SOS1基因可能在镰刀草耐盐性中起重要作用,对其他作物的耐盐性也有一定的指导意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation and characterization of plasma membrane Na7H+ antiporter (SOS1) gene during salinity stress in kallar grass {Leptochloa fusca)
Background: Leptochloa fusca is a halophyte plant which is highly tolerant to saline and sodic soils and water. Moreover, L fusca is an attractive model plant to study the mechanism of salt tolerance mainly due to its characteristics as a typical euhalophyte, having both accumulating and excreting salt properties. Soil salinity adversely affects plant growth, development and disturbs intracellular ion homeostasis resulting cellular toxicity. The Salt Overly Sensitive 1 (SOS1) gene encodes a plasma membrane NaVH+ antiporter that plays an important role in imparting salt stress tolerance of plants. Material and Methods: Using conserved sequences of S O S 1 , the coding sequence of plasma membrane NaVH+ antiporter (SOS1) in kallar grass was partially isolated and its expression profile during salinity stress was investigated. Results: The aa (amino acid) sequence of the isolated region of /./SOS1 possesses the maximum identity up to 96% of its orthologue in Distichlis spicata. The results of semi-quantitative RT-PCR revealed that salinization was affected SOS1 transcript level positively. The expression of i./50S1 in leaves of kallar grass progressively increased under all salinity levels compared to control. Conclusions: The results suggest that i./SOS1 may play an essential role in the salt tolerance of L fusca and may be useful for improving salt tolerance in other crop species.
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来源期刊
Eurasian Journal of Biosciences
Eurasian Journal of Biosciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
1.20
自引率
0.00%
发文量
0
期刊介绍: EurAsian Journal of BioSciences (Abbrev. Eurasia J Biosci or EJOBIOS) is an international, refereed electronic journal. It publishes the results of original research in the field of biological sciences restricted tomorphology, physiology, genetics, taxonomy, ecology and biogeography of both prokaryotic and eucaryotic organisms. The journal encourages submission of manuscripts dealing with plant biology, animal biology, plant physiology, microbiology, hydrobiology, ecology and environmental science, ethnobiology, biodiversity and conservation biology. EurAsian Journal of BioSciences publishes original articles in the following areas: -Agriculture, Fisheries & Food -Anatomy & Morphology -Behavioural Sciences -Biology, Biochemistry and Biotechnology -Biophysics -Biology Education -Cellular Biology and Anatomical Sciences -Ecology, Evolution & Environment -Entomology -Forestry -General Biology -Genetics & Heredity -Life Sciences - Other topics -Microbiology and Immunology -Molecular Biology -Mycology -Palaeontology -Parasitology -Pharmacology & Pharmacy -Physiology and Related Sciences -Plant Sciences -Toxicology -Veterinary Sciences -Virology -Zoology
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