BAP对土耳其东部安纳托利亚地区特有种金丝桃芽培养中金丝桃素总产量的影响

Q3 Agricultural and Biological Sciences
Hilal Surmuş Asan, H. Özen, A. Onay, N. Asan
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引用次数: 4

摘要

背景:由于金丝桃素在治疗中的重要性,许多金丝桃属植物正在被研究。本研究的目的是确定不同浓度的BAP(6-苄基氨基嘌呤)对土耳其东安纳托利亚地区特有的金丝桃(Hypericum scabroides)种子萌发、芽增殖和总金丝桃素的影响。材料与方法:取自土耳其东部安纳托利亚(Elazig)的金丝桃标本。采用组织培养法测定了不同浓度BAP(0.0(对照)、0.5、1.0和2.0 mg/L)对金丝桃种子萌发、芽部增殖和总金丝桃素积累的影响。萌发和芽部培养采用MS培养基。用紫外分光光度计测定金丝桃素的含量。结果:无激素MS培养基(对照组)萌发率最高,为59.2%。将刚发芽的幼苗的根尖在添加不同BAP浓度(0.5、1.0和2.0 mg/L)的MS培养基和不添加BAP的对照组上增殖。在添加2.0 mg/L BAP的MS培养基上,芽数最多(42.7个芽/外植体),芽长最长(2.50 cm)。结果表明,总金丝桃素的含量不受BAP浓度的影响。结论:BAP浓度的增加促进了金丝桃芽的增殖,但对金丝桃种子发芽率和总金丝桃素含量没有影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of BAP on total hypericin production in shoot cultures of Hypericum scabroides: An endemic species in the Eastern Anatolia Region of Turkey
Background: Due to the therapeutic importance of hypericin, a number of Hypericum species are being investigated. The aim of this study was to determine the effect of various BAP (6- benzylaminopurine) concentrations on seed germination, shoot proliferation, and total hypericin in a tissue culture of Hypericum scabroides which is endemic to the Eastern Anatolia region, Turkey. Material and Methods: Hypericum scabroides specimens were collected from Eastern Anatolia (Elazig, Turkey). The effects of various BAP concentrations (0.0 (control), 0.5, 1.0, and 2.0 mg/L) on seed germination, shoot multiplication, and the accumulation of total hypericin were determined using tissue cultures of H. scabroides. Murashige and Skoog (MS) medium was used for germination and shoot cultures. Measurements of total hypericin were taken using a UV spectrophotometer. Results: The best germination rate (59.2%) was obtained using hormone-free MS medium (control group). Apical tips of freshly germinated seedlings were proliferated on the MS medium supplemented with various BAP concentrations (0.5, 1.0, and 2.0 mg/L) and the control group (without BAP). The highest number of shoots (42.7 shoot/explant) and longest shoot length (2.50 cm) were obtained on MS medium supplemented with 2.0 mg/L BAP. Total hypericin was found in trace amounts and it was found that the total hypericin was not affected by the concentration of BAP. Conclusions: Our results showed that increasing concentrations of BAP stimulated shoot multiplication but did not affect seed germination rates or total hypericin in in vitro cultures of H. scabroides.
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来源期刊
Eurasian Journal of Biosciences
Eurasian Journal of Biosciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
1.20
自引率
0.00%
发文量
0
期刊介绍: EurAsian Journal of BioSciences (Abbrev. Eurasia J Biosci or EJOBIOS) is an international, refereed electronic journal. It publishes the results of original research in the field of biological sciences restricted tomorphology, physiology, genetics, taxonomy, ecology and biogeography of both prokaryotic and eucaryotic organisms. The journal encourages submission of manuscripts dealing with plant biology, animal biology, plant physiology, microbiology, hydrobiology, ecology and environmental science, ethnobiology, biodiversity and conservation biology. EurAsian Journal of BioSciences publishes original articles in the following areas: -Agriculture, Fisheries & Food -Anatomy & Morphology -Behavioural Sciences -Biology, Biochemistry and Biotechnology -Biophysics -Biology Education -Cellular Biology and Anatomical Sciences -Ecology, Evolution & Environment -Entomology -Forestry -General Biology -Genetics & Heredity -Life Sciences - Other topics -Microbiology and Immunology -Molecular Biology -Mycology -Palaeontology -Parasitology -Pharmacology & Pharmacy -Physiology and Related Sciences -Plant Sciences -Toxicology -Veterinary Sciences -Virology -Zoology
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