生物燃料作物麻疯树叶绿体微卫星和ISSR标记的研究

Q3 Agricultural and Biological Sciences
N. Mittal, A. Dubey
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引用次数: 10

摘要

鉴定出一组新的ISSR多态性和叶绿体微卫星标记,用于标记麻疯树不同种质间的亲缘关系。利用12条多态性ISSR引物,从10个麻瓜种质中共扩增出26个片段。微卫星基因座的跨种扩增是开发新物种基因座特异性标记的一种节省时间和成本的方法。此外,为了揭示菖蒲(Acorus calamus)叶绿体微卫星引物对在不同种质间的高度多态性和遗传变异,对不同种质间的叶绿体微卫星引物对进行了跨种扩增和验证。在18个cpSSRs中,鉴定出6个(33.33%)具有良好的扩增和多态性。每对cpSSR引物扩增的等位基因数在2 ~ 4个之间,共有18个大小变异。10份材料中6个位点的18个大小变异组合成4个不同的单倍型。总叶绿体DNA多样性(HT)和分化率(GST)分别为0.700和0.560。利用ISSR和cpSSR标记估算麻树不同种质间的Shannon’s信息指数(I)、Nei’s遗传多样性为0.33±0.28;0.22±0.19和0.94±0.35;分别为0.55±0.18。采用UPGMA聚类分析方法确定了各种质间的遗传关系,并将其划分为2个主要聚类:聚类1和聚类2。讨论了这些结果在设计一套鉴定的新标记对麻树遗传关系和遗传多样性分析的重要性方面的意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A novel set of highly polymorphic chloroplast microsatellite and ISSR markers for the biofuel crop Jatropha curcas
A novel set of polymorphic ISSR and chloroplast microsatellite markers were identified to mark the genetic relationship among different accessions of Jatropha curcas. A total of 12 polymorphic ISSR primers used, amplified a total of 26 fragments among ten different accessions of J. curcas. The cross-species amplification of microsatellite loci is timesaving as well as a cost-effective approach for developing locus specific markers for new species. Further, in an attempt to reveal a high degree of polymorphism and genetic variation among different accessions of J. curcas chloroplast microsatellite primer pairs developed for Acorus calamus (Acoraceace) were examined for cross-species amplification and validation among them. Out of 18 cpSSRs, six (33.33%) were identified as resulting in good amplifications and polymorphism. The number of amplified alleles per cpSSR primer pair ranged from 2 to 4 with the total number of 18 size variants as whole. The 18 size variants at 6 loci among 10 accessions were combined in 4 different haplotypes. The total chloroplast DNA diversity (HT) and the differentiation (GST) were found as 0.700 and 0.560 respectively. Whereas, Shannon's information index (I), Nei's genetic diversity among different accessions of J. curcas using ISSR and cpSSR markers was estimated at 0.33±0.28; 0.22±0.19 and 0.94±0.35; 0.55±0.18 respectively. The genetic relationship among the accessions was established using UPGMA cluster analysis, which were grouped into two major clusters denoted as cluster-I and cluster-II. The implication of the results in devising the importance of a identified novel set of markers for the genetic relationship and genetic diversity analysis in J. curcas is discussed.
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来源期刊
Eurasian Journal of Biosciences
Eurasian Journal of Biosciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
1.20
自引率
0.00%
发文量
0
期刊介绍: EurAsian Journal of BioSciences (Abbrev. Eurasia J Biosci or EJOBIOS) is an international, refereed electronic journal. It publishes the results of original research in the field of biological sciences restricted tomorphology, physiology, genetics, taxonomy, ecology and biogeography of both prokaryotic and eucaryotic organisms. The journal encourages submission of manuscripts dealing with plant biology, animal biology, plant physiology, microbiology, hydrobiology, ecology and environmental science, ethnobiology, biodiversity and conservation biology. EurAsian Journal of BioSciences publishes original articles in the following areas: -Agriculture, Fisheries & Food -Anatomy & Morphology -Behavioural Sciences -Biology, Biochemistry and Biotechnology -Biophysics -Biology Education -Cellular Biology and Anatomical Sciences -Ecology, Evolution & Environment -Entomology -Forestry -General Biology -Genetics & Heredity -Life Sciences - Other topics -Microbiology and Immunology -Molecular Biology -Mycology -Palaeontology -Parasitology -Pharmacology & Pharmacy -Physiology and Related Sciences -Plant Sciences -Toxicology -Veterinary Sciences -Virology -Zoology
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