{"title":"大肠杆菌和朱尼不动杆菌在不同浓度氯化钠下的存活率","authors":"J. Hrenović, T. Ivankovic","doi":"10.5053/EJOBIOS.2009.3.0.18","DOIUrl":null,"url":null,"abstract":"The survival of two heterotrophic bacteria in conditions of various concentrations of NaCl was tested. Both bacteria can commonly enter marine environments through sewage or wastewater treatment plant effluents; the Escherichia coli is a common enteric bacterium and Acinetobacter junii is a phosphate-accumulating bacterium inhabiting activated sludge. When cultivated in nutrient rich media (COD 8700 mg O2 L -1 ), both bacteria were multiplying during 72 h at concentrations of NaCl up to 5% for E. coli and 3.5% for A. junii. Total die-off of E. coli was detected at 72 h by NaCl concentration of 20%. Total die-off of A. junii was detected at 72 h by NaCl concentration of 10%. When the same bacteria were cultured in nutrient depleted media (COD 90 mg O2 L -1 ), the multiplication of E. coli stopped at 3.5% of NaCl and higher, but the cells were able to survive for longer period of time at extreme NaCl concentrations of 20 and 30%. The negative influence of NaCl to A. junii was pronounced in conditions of organic matter shortage and rapid die-off was observed at 3.5% of NaCl and higher. Both bacteria seemed to be osmotolerant when cultured in nutrient-rich media, but not in nutrient-depleted media.","PeriodicalId":11848,"journal":{"name":"Eurasian Journal of Biosciences","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2009-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5053/EJOBIOS.2009.3.0.18","citationCount":"52","resultStr":"{\"title\":\"Survival of Escherichia coli and Acinetobacter junii at various concentrations of sodium chloride\",\"authors\":\"J. Hrenović, T. Ivankovic\",\"doi\":\"10.5053/EJOBIOS.2009.3.0.18\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The survival of two heterotrophic bacteria in conditions of various concentrations of NaCl was tested. Both bacteria can commonly enter marine environments through sewage or wastewater treatment plant effluents; the Escherichia coli is a common enteric bacterium and Acinetobacter junii is a phosphate-accumulating bacterium inhabiting activated sludge. When cultivated in nutrient rich media (COD 8700 mg O2 L -1 ), both bacteria were multiplying during 72 h at concentrations of NaCl up to 5% for E. coli and 3.5% for A. junii. Total die-off of E. coli was detected at 72 h by NaCl concentration of 20%. Total die-off of A. junii was detected at 72 h by NaCl concentration of 10%. When the same bacteria were cultured in nutrient depleted media (COD 90 mg O2 L -1 ), the multiplication of E. coli stopped at 3.5% of NaCl and higher, but the cells were able to survive for longer period of time at extreme NaCl concentrations of 20 and 30%. The negative influence of NaCl to A. junii was pronounced in conditions of organic matter shortage and rapid die-off was observed at 3.5% of NaCl and higher. Both bacteria seemed to be osmotolerant when cultured in nutrient-rich media, but not in nutrient-depleted media.\",\"PeriodicalId\":11848,\"journal\":{\"name\":\"Eurasian Journal of Biosciences\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2009-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.5053/EJOBIOS.2009.3.0.18\",\"citationCount\":\"52\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Eurasian Journal of Biosciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5053/EJOBIOS.2009.3.0.18\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Eurasian Journal of Biosciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5053/EJOBIOS.2009.3.0.18","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 52
摘要
研究了两种异养菌在不同NaCl浓度条件下的存活率。这两种细菌通常可以通过污水或污水处理厂的流出物进入海洋环境;大肠杆菌是一种常见的肠道细菌,而朱尼不动杆菌是一种寄生在活性污泥中的磷酸盐积累菌。当在富营养化培养基(COD 8700 mg O2 L -1)中培养时,两种细菌在NaCl浓度分别为5%和3.5%时均能繁殖72 h。用浓度为20%的NaCl对大肠杆菌进行72 h的总死亡检测。在NaCl浓度为10%的条件下,对刺槐进行72 h的总死亡检测。当同样的细菌在缺乏营养的培养基(COD为90 mg O2 L -1)中培养时,当NaCl浓度为3.5%或更高时,大肠杆菌的增殖停止,但在极端NaCl浓度为20%和30%时,细胞能够存活更长的时间。在有机质不足的条件下,NaCl对刺槐的影响显著,在3.5%及以上NaCl处理下,刺槐快速死亡。两种细菌在营养丰富的培养基中培养时似乎都具有渗透性,而在营养贫乏的培养基中则没有。
Survival of Escherichia coli and Acinetobacter junii at various concentrations of sodium chloride
The survival of two heterotrophic bacteria in conditions of various concentrations of NaCl was tested. Both bacteria can commonly enter marine environments through sewage or wastewater treatment plant effluents; the Escherichia coli is a common enteric bacterium and Acinetobacter junii is a phosphate-accumulating bacterium inhabiting activated sludge. When cultivated in nutrient rich media (COD 8700 mg O2 L -1 ), both bacteria were multiplying during 72 h at concentrations of NaCl up to 5% for E. coli and 3.5% for A. junii. Total die-off of E. coli was detected at 72 h by NaCl concentration of 20%. Total die-off of A. junii was detected at 72 h by NaCl concentration of 10%. When the same bacteria were cultured in nutrient depleted media (COD 90 mg O2 L -1 ), the multiplication of E. coli stopped at 3.5% of NaCl and higher, but the cells were able to survive for longer period of time at extreme NaCl concentrations of 20 and 30%. The negative influence of NaCl to A. junii was pronounced in conditions of organic matter shortage and rapid die-off was observed at 3.5% of NaCl and higher. Both bacteria seemed to be osmotolerant when cultured in nutrient-rich media, but not in nutrient-depleted media.
期刊介绍:
EurAsian Journal of BioSciences (Abbrev. Eurasia J Biosci or EJOBIOS) is an international, refereed electronic journal. It publishes the results of original research in the field of biological sciences restricted tomorphology, physiology, genetics, taxonomy, ecology and biogeography of both prokaryotic and eucaryotic organisms. The journal encourages submission of manuscripts dealing with plant biology, animal biology, plant physiology, microbiology, hydrobiology, ecology and environmental science, ethnobiology, biodiversity and conservation biology. EurAsian Journal of BioSciences publishes original articles in the following areas: -Agriculture, Fisheries & Food -Anatomy & Morphology -Behavioural Sciences -Biology, Biochemistry and Biotechnology -Biophysics -Biology Education -Cellular Biology and Anatomical Sciences -Ecology, Evolution & Environment -Entomology -Forestry -General Biology -Genetics & Heredity -Life Sciences - Other topics -Microbiology and Immunology -Molecular Biology -Mycology -Palaeontology -Parasitology -Pharmacology & Pharmacy -Physiology and Related Sciences -Plant Sciences -Toxicology -Veterinary Sciences -Virology -Zoology