黑森蝇(Mayetiola destructor, Say)唾液分泌蛋白变异与毒力的关系

Y. M. Crane, C. Crane, B. Schemerhorn
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引用次数: 1

摘要

唾液蛋白是定居害虫与其寄主植物之间的最初接触。推测一种或多种唾液蛋白介导黑森蝇与小麦的相互作用,从而建立有利于黑森蝇的取食场所。对分布于8种黑线蝇(B、C、D、E、GP、L、O和vH9)的384个个体的52个昆虫分泌唾液蛋白位点进行了调查。扩增子用Illumina测序,序列读段与设计引物的参考序列对齐。鉴定出碱基一致变异位点(共998个)并按生物型制表。对小麦抗性基因H3、H5、H6、H7/H8、H9、H11、H13和H26的全生物型毒力与位置变化无关。本研究中使用的多板池策略是一种有效且经济的方法,可以揭示数百个个体在数十个遗传位点上的基因型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Relationship of Secreted Salivary Protein Variants to Virulence in Hessian Fly (Mayetiola destructor (Say))
Salivary proteins are the initial contact between sedentary insect pests and their host plants. It is expected that one or more salivary proteins mediate the interaction between Hessian fly and wheat, in which a feeding site is established to the benefit of the fly. A survey of 52 loci annotated as insect secreted salivary proteins was conducted in 384 individuals evenly distributed among eight biotypes of Hessian fly (B, C, D, E, GP, L, O, and vH9). Amplicons were sequenced with Illumina, and sequence reads were aligned to the reference sequences from which primers had been designed. Positions of consistent base variation (998 in all) were identified and tabulated by biotype. No varying position was associated with biotype-wide virulence to any one of wheat resistance genes H3, H5, H6, H7/H8, H9, H11, H13, and H26. The multiplate pooling strategy utilized in this study is an effective, affordable way to reveal the genotype of hundreds of individuals at tens of genetic loci.
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