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引用次数: 42
摘要
结核病是一种危及生命的疾病，由结核分枝杆菌引起。高度耐药的出现和结核分枝杆菌(MOTT)的流行要求寻找新的抗结核药物。在这种情况下，银纳米颗粒(AgNPs)以其抗菌活性而闻名，通过微孔板Alamar Blue Assay (MABA)对临床分离的结核分枝杆菌多重耐药(MDR)、广泛耐药(XDR)和MOTT菌株进行了评估。临床分离株均在AgNPs浓度为6.25 ~ 12.5 μg/ml范围内受到抑制。利用从匍匐花中提取的酶对硝酸银(AgNO3)溶液进行生物还原合成纳米银，并用紫外-可见分光光度法、FTIR、XRD、AFM等方法对其进行了表征。合成了平均粒径为5 nm的球形AgNPs，并对其抑菌活性进行了评价。
Biosynthesis of monodispersed silver nanoparticles and their activity against Mycobacterium tuberculosis
Tuberculosis (TB) is a life threatening disease, caused by Mycobacterium tuberculosis. Emergence of high degree of drug-resistance and prevalence of Mycobacterium other than tuberculosis (MOTT) necessitates the search for new anti-tubercular agents. In that context, silver nanoparticles (AgNPs) which have been known for their antimicrobial activity were evaluated against clinical isolates of M. tuberculosis Multi-Drug Resistant (MDR), Extensive-Drug Resistant (XDR) and MOTT strains, through Microplate Alamar Blue Assay (MABA). All the clinical isolates got inhibited within the MIC range of 6.25 to 12.5 μg/ml of AgNPs. Silver nanoparticles were synthesized by bioreduction of silver nitrate (AgNO3) solution with enzymes extracted from R. stolonifer and characterized using UV-Vis absorption spectrophotometry, FTIR, XRD, AFM. A spherical shaped AgNPs with the average particle size of 5 nm were synthesized and evaluated for their antimycobacterial activity.
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