硫酸粘菌素对小鼠坐骨-胫神经毒性作用的电生理变化

Q4 Pharmacology, Toxicology and Pharmaceutics

中国药理学与毒理学杂志 Pub Date : 2012-12-01 DOI:10.3867/J.ISSN.1000-3002.2012.06.012

Chongshan Dai, Ji Chang Li, Wei Lin, Feng Wang, M. C. Sun, Jian Li

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Sun, Jian Li","doi":"10.3867/J.ISSN.1000-3002.2012.06.012","DOIUrl":null,"url":null,"abstract":"OBJECTIVE To investigate the toxic actions of colistin sulphate on sciatic-tibial nerves of mice.METHODS Colistin sulphate 7.5 mg·kg-1 was iv administered to Kunming female mice,twice a day.The mice were divided into colistin sulphate 1,3,7 d and 7 d post colistin sulphate groups according the adminstration for 1,3 and 7 d,respectively.The body mass and gait observations were obtained first,followed by determinations of foot splay and the threshold intensity(TI),maximal intensity(MI),compound action potential duration(CAPD),latency of compound action potential(CAPL),compound action potential amplitude(peak to peak)(CAPA),and nerve conduction velocity(NCV) of sciatic-tibial nerve.Serum creatinine(Cre) and urea nitrogen(BUN) concentrations were determined before the first dose on 2nd day(d 2),d 4,d 8 and d 15(the day of the first dose as the first day).RESULTS Compared with normal control group,after colistin sulphate was given for 1 d,Cre showed significant difference(P0.05);and for 3 d,BUN and body mass showed significant difference,respectively(P0.05);after colistin sulphate was given for 7 d,the gait and the foot splay showed significant difference(both P0.05).Compared with normal control group,after colistin sulphate was given for 1,3 and 7 d,TI increased by 60%,60% and 192%(P0.01),respectively;MI increased by 4%,13% and 100%(P0.05),respectively;CAPD increased by 15.1%,11.5% and 52.8%(P0.05),respectively;CAPL prolonged by 9.0%,9.0% and 14.6%(P0.05),respectively;CAPA decreased 0.92%,16.2% and 47.6%(P0.01),respectively;and NCV decreased by 7.6%,7.5% and 12.7%(P0.05),respectively.After 7 d of stopping administration,the gait and the foot splay had no significant differences,and all electrophysiology indexes showed a recovery tendency and only CAPA was significantly different(P0.05).CONCLUTION Nephrotoxicity is earlier than neurotoxicity on mice treated with colistin sulphate,at the same time,the progressive changes in CAP characteristics of the sciatic-tibial nerves are dependent with the time of colistin sulphate administration and abnormal CAPA has the longest duration time.","PeriodicalId":10149,"journal":{"name":"中国药理学与毒理学杂志","volume":"26 1","pages":"847-852"},"PeriodicalIF":0.0000,"publicationDate":"2012-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Electrophysiological changes for toxic effect of colistin sulphate on sciatic-tibial nerves in mice\",\"authors\":\"Chongshan Dai, Ji Chang Li, Wei Lin, Feng Wang, M. 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引用次数: 0

摘要

目的探讨硫酸粘菌素对小鼠坐骨-胫神经的毒性作用。方法昆明雌性小鼠静脉注射硫酸粘菌素7.5 mg·kg-1，每天2次。小鼠按给药1、3、7 d分为硫酸粘菌素后1、3、7 d组。首先进行体质量和步态观察，然后测定足张度和阈值强度(TI)、最大强度(MI)、复合动作电位持续时间(CAPD)、复合动作电位潜伏期(CAPL)、复合动作电位振幅(峰对峰)(CAPA)、坐骨胫神经神经传导速度(NCV)。测定首次给药前第2天(第2天)、第4天、第8天、第15天(第1次给药当天)血清肌酐(Cre)和尿素氮(BUN)浓度。结果与正常对照组比较，给予硫酸粘菌素1 d后，Cre差异有统计学意义(P0.05);给予硫酸粘菌素3 d后，BUN、体质量差异有统计学意义(P0.05);给予硫酸粘菌素7 d后，步态、足展度差异有统计学意义(P0.05)。与正常对照组比较，给予硫酸粘菌素1、3、7 d后，TI分别提高了60%、60%、192%(P0.01)， MI分别提高了4%、13%、100%(P0.05)， CAPD分别提高了15.1%、11.5%、52.8%(P0.05)， CAPL分别延长了9.0%、9.0%、14.6%(P0.05)， CAPA分别降低了0.92%、16.2%、47.6%(P0.01)， NCV分别降低了7.6%、7.5%、12.7%(P0.05)。停药7 d后，步态和足展无显著差异，各电生理指标均有恢复趋势，仅CAPA有显著差异(P0.05)。结论硫酸粘菌素对小鼠的肾毒性早于神经毒性，同时，坐骨胫神经CAP特征的进行性改变与硫酸粘菌素给药时间有关，且CAPA异常持续时间最长。

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Electrophysiological changes for toxic effect of colistin sulphate on sciatic-tibial nerves in mice

OBJECTIVE To investigate the toxic actions of colistin sulphate on sciatic-tibial nerves of mice.METHODS Colistin sulphate 7.5 mg·kg-1 was iv administered to Kunming female mice,twice a day.The mice were divided into colistin sulphate 1,3,7 d and 7 d post colistin sulphate groups according the adminstration for 1,3 and 7 d,respectively.The body mass and gait observations were obtained first,followed by determinations of foot splay and the threshold intensity(TI),maximal intensity(MI),compound action potential duration(CAPD),latency of compound action potential(CAPL),compound action potential amplitude(peak to peak)(CAPA),and nerve conduction velocity(NCV) of sciatic-tibial nerve.Serum creatinine(Cre) and urea nitrogen(BUN) concentrations were determined before the first dose on 2nd day(d 2),d 4,d 8 and d 15(the day of the first dose as the first day).RESULTS Compared with normal control group,after colistin sulphate was given for 1 d,Cre showed significant difference(P0.05);and for 3 d,BUN and body mass showed significant difference,respectively(P0.05);after colistin sulphate was given for 7 d,the gait and the foot splay showed significant difference(both P0.05).Compared with normal control group,after colistin sulphate was given for 1,3 and 7 d,TI increased by 60%,60% and 192%(P0.01),respectively;MI increased by 4%,13% and 100%(P0.05),respectively;CAPD increased by 15.1%,11.5% and 52.8%(P0.05),respectively;CAPL prolonged by 9.0%,9.0% and 14.6%(P0.05),respectively;CAPA decreased 0.92%,16.2% and 47.6%(P0.01),respectively;and NCV decreased by 7.6%,7.5% and 12.7%(P0.05),respectively.After 7 d of stopping administration,the gait and the foot splay had no significant differences,and all electrophysiology indexes showed a recovery tendency and only CAPA was significantly different(P0.05).CONCLUTION Nephrotoxicity is earlier than neurotoxicity on mice treated with colistin sulphate,at the same time,the progressive changes in CAP characteristics of the sciatic-tibial nerves are dependent with the time of colistin sulphate administration and abnormal CAPA has the longest duration time.

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来源期刊

中国药理学与毒理学杂志 Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

0.20

自引率

0.00%

发文量

6787

期刊介绍： Chinese Journal of Pharmacology and Toxicology was first published in November 1986. As a journal of high academic value jointly edited by the Chinese Pharmacological Society, Chinese Society of Toxicology and the Academy of Military Medical Sciences (AMMS), it is a dominating one in China’s biomedicine, devoted to publication of research findings, reviews of special topic and new technologies and methods in every branch of experimental toxicology and pharmacology.