Yeni Bir Rinosinüzit Modeli Geliştirmek: Bir Hayvan Deneyi

Aim: To develop a new model of acute bacterial rhinosinusitis. Method: We divided guinea pigs into four groups in this study. In the primary group of guinea pigs, right nasal cavities of the animals were vaccinated with Streptococcus pneumoniae suspension. In the second group, sponge strips saturated with sterile saline into the right nasal cavities of animals. In the third group, sponge strips saturated with Streptococcus pneumoniae suspension in the right nasal cavities of animals. The fourth group was control group, which was exposed to no intervention. After 7, 14, 28, 35 days from interventions, intranasal cultures were obtained, computed tomography (CT) scans were imaged from all of the guinea pigs, and two randomly selected guinea pigs that were detected as having rhinosinusitis radiologically were killed every week. The sinuses and nasal specimens of sacrificed guinea pigs were prepared for histopathological investigation. Results: Radiological and histopathological examinations of nasal samples were performed to observe the severity of the inflammatory reaction. Acute bacterial rhinosinusitis was induced in all groups of subject animals except the control group. More severe inflammation was seen in the third group of subject animals compared to the first and second groups. No inflammatory reaction was found in the control group. We also evaluated the normal flora of guinea pigs. Conclusion: The guinea pig is certainly a true model for developing rhinosinusitis. Guinea pigs should be considered as an alternative model for further potential studies of genetics and inflammation, even though surgical manipulation is limited.