肝组织病理变化的偏振光可视化

IF 0.7 4区物理与天体物理 Q4 OPTICS

Optica Applicata Pub Date : 2022-01-01 DOI:10.37190/oa220306

A. Stoilova, B. Blagoeva, D. Nazarova, E. Stoykova, N. Berberova-Buhova, L. Nedelchev, A. Machikhin

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引用次数: 0

摘要

在这项工作中，我们应用了两种基于偏振光的方法来可视化肝脏病理的组织学模式。第一种方法是通过偏光显微镜获取两幅图像，其中一幅图像(Ppar)是由平行于照明偏振方向的分析仪获得的，另一幅图像(Pper)是由垂直于照明方向的分析仪获得的。最终的图像是基于极化比，preconstruct = (Ppar - Pper)/(Ppar + Pper)。第二种方法是用波长为635 nm的偏振激光束照射组织学标本。测量了方位角、椭圆角、偏振度和反射功率等偏振参数，量化了入射光与健康组织和形态异常组织样品相互作用后偏振状态的变化。

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Visualization of pathologic changes in liver tissue via polarized light

In this work, we applied two polarized light based approaches to visualize histological patterns of liver pathologies. The first one involves acquisition of two images through a polarizing microscope, one image (Ppar) acquired with the analyzer oriented parallel to the polarization of illumination and the other (Pper) acquired with the analyzer oriented perpendicular to the illumination. The final image is based on the polarization ratio, Preconstructed = (Ppar – Pper)/(Ppar + Pper). Using the second technique, the histological specimens were illuminated with a polarized laser beam with wavelength of 635 nm. Polarimetric parameters as azimuth, angle of ellipticity, degree of polarization and reflected power have been measured to quantify the change in the polarization state of the incident light after interaction with the sample of the healthy tissue and of the tissue with abnormal morphological changes.

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来源期刊

Optica Applicata 物理-光学

CiteScore

1.00

自引率

16.70%

发文量

审稿时长

4 months

期刊介绍： Acoustooptics, atmospheric and ocean optics, atomic and molecular optics, coherence and statistical optics, biooptics, colorimetry, diffraction and gratings, ellipsometry and polarimetry, fiber optics and optical communication, Fourier optics, holography, integrated optics, lasers and their applications, light detectors, light and electron beams, light sources, liquid crystals, medical optics, metamaterials, microoptics, nonlinear optics, optical and electron microscopy, optical computing, optical design and fabrication, optical imaging, optical instrumentation, optical materials, optical measurements, optical modulation, optical properties of solids and thin films, optical sensing, optical systems and their elements, optical trapping, optometry, photoelasticity, photonic crystals, photonic crystal fibers, photonic devices, physical optics, quantum optics, slow and fast light, spectroscopy, storage and processing of optical information, ultrafast optics.