补充牛乳铁蛋白对早期坏死性小肠结肠炎模型大鼠肠道炎症因子表达的影响

Q4 Medicine

中华实用儿科临床杂志 Pub Date : 2020-03-13 DOI:10.3760/CMA.J.ISSN.2095-428X.2020.02.013

Xiang-yong Kong, Lu Zhuang, Meiyu Wang, Shan Zhang, Jianru Peng, Zhichun Feng

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引用次数: 0

摘要

目的探讨补充牛乳铁蛋白(bLF)对坏死性小肠结肠炎(NEC)模型大鼠肠黏膜组织的保护作用及其对炎症因子的影响，为补充牛乳铁蛋白预防NEC提供理论依据。方法将SD大鼠随机分为4组，每组25只。对照组:口服喂养;模型组:口服脂多糖灌胃+缺氧刺激;高剂量bLF干预组:每日bLF (7 g/L) +口服灌胃+ LPS灌胃+缺氧刺激;低剂量bLF干预组:每日bLF (2g /L) +口服灌胃+ LPS灌胃+缺氧刺激。HE染色进行组织病理学分析。采用酶联免疫吸附法(ELISA)检测肠黏膜组织中白细胞介素-1β(IL-1β)和白细胞介素-6(IL-6)的表达水平。结果(1)形态学观察:模型组大鼠肠壁较薄，肠腔内有不同程度尘肺及积液。镜下可见肠组织坏死严重，肠绒毛脱落，腺体排列紊乱，上皮水肿明显，固有层与粘膜下层严重水肿分离，有大量炎性细胞浸润。(2)肠组织中IL-1β和IL-6的表达:模型组大鼠IL-1β和IL-6的组织浓度[(380.89±20.25)ng/L，(485.12±31.44)ng/L]显著高于对照组[(270.69±45.58)ng/L，(212.62±89.46)ng/L](q =9.785, 14.030，均P 0.05;IL-6: q=-1.140, P < 0.05)。结论肠内添加bLF可减轻早产SD大鼠NEC模型肠组织损伤，抑制肠组织中IL-1β和IL-6炎症因子的表达，对肠组织具有保护作用。关键词:牛乳铁蛋白;坏死性小肠结肠炎;Interleukin-1β;白细胞介素- 6

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Effect of bovine lactoferrin supplementation on intestinal inflammatory factor expression in premature rats model of necrotizing enterocolitis

Objective To investigate the protective effects of bovine lactoferrin (bLF) supplementation on intestinal mucosal tissue and its influence on of inflammatory factors in the premature rats model of necrotizing enterocolitis(NEC), and to provide the theoretical basis for prevention of NEC by bLF supplementation. Methods Premature SD rats were randomly divided into 4 groups, 25 cases in each group.Control group: oral feeding; model group : oral feeding with lipopolysaccharides(LPS) gavage + hypoxic stimulation; high dose bLF intervention group: daily bLF (7 g/L) + oral feeding with LPS gavage + hypoxic stimulation; low dose bLF intervention group: daily bLF (2 g/L) + oral feeding with LPS gavage + hypoxic stimulation.Histopathological analysis was performed by HE staining.The expression levels of interleukin-1β(IL-1β)and interleukin-6(IL-6)in intestinal mucosa were detected by enzyme linked immunosorbent assay (ELISA). Results (1) Morphological observation: the intestinal wall of model group was thin, and there were different degrees of pneumoconiosis and effusion in intestinal cavity.Under the microscopy, it could be observed that the intestinal tissue necrosis was serious, the intestinal villi fell off, glands arranged disorderly, epithelial edema was significant, the lamina propria and submucosa had severely edema and were separated, and there were a large number of inflammatory cells infiltrated.The above-mentioned manifestations were alleviated in the high-dose and low-dose bLF intervention groups, and no significant abnormalities were found in the control group.(2) The expression of IL-1β and IL-6 in intestinal tissue: the tissue concentration of IL-1β and IL-6 in the model group rats [(380.89±20.25) ng/L, (485.12±31.44) ng/L]were significantly higher than those in the control group[(270.69±45.58) ng/L, (212.62±89.46) ng/L](q =9.785, 14.030, all P 0.05; IL-6: q=-1.140, P>0.05). Conclusion Enteral bLF supplementation can alleviate the damage of intestinal tissue in NEC model of premature SD rats, inhibit the expression of IL-1β and IL-6 inflammatory factors in intestinal tissue, and have a protective effect on intestinal tissue. Key words: Bovine lactoferrin; Necrotizing enterocolitis; Interleukin-1β; Interleukin-6

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来源期刊

中华实用儿科临床杂志 Medicine-Pediatrics, Perinatology and Child Health

CiteScore

0.60

自引率

0.00%

发文量

14243

期刊介绍： Chinese Journal of Applied Clinical Pediatrics ( semi-monthly ) is a core journal of paediatrics under the supervision of China Association for Science and Technology, sponsored by Chinese Medical Association and undertaken by Xinxiang Medical College. Founded in 1986, it is openly circulated both at home and abroad. The journal has several columns, such as Expert Forum, Experimental Research and Paediatric Surgery, which are mainly for paediatric medical workers and medical researchers in hospitals. Its purpose is to reflect the new theories and technologies in paediatric medicine and scientific research at home and abroad, and to promote academic exchanges. Chinese Journal of Applied Clinical Pediatrics is a source journal of China Science Citation Database (CSCD), a core journal of Peking University, a source journal of Chinese science and technology paper statistics (China Science and Technology Core Journals), a core academic journal of RCCSE, a high-quality scientific and technical journal of China, a high-quality scientific and technical journal of China Association for Science and Technology, and a high-quality scientific and technical journal of China Biomedical Science and Technology Association. We have been published in China Biomedical Literature Database (SinoMed), China Knowledge Network, Wanfang Data Knowledge Service Platform, China Academic Journal Abstracts, Scopus Database, Chemical Abstracts (USA), Japan Science and Technology Agency (JSTA) Database, Copernicus Abstracts (Poland), Abstracts of the Centre for Agricultural and Biological Sciences (CABS) of the United Kingdom, Cambridge Scientific Abstracts ProQuest Database, WHO Medical Journal of the Western Pacific Region (WMPR), and WHO Medical Journal of the Western Pacific Region (WMPR) of the United States. We have been included in dozens of authoritative databases at home and abroad, such as WHO Western Pacific Region Index of Medicine (WPRIM), Ullrich's Guide to Periodicals, and so on.