低温保存对河蟹精子酶活性的影响

Q4 Environmental Science
Yunhui Pu, X. Xu, Huan Gao, Zhao-pu Liu, B. Yan
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引用次数: 0

摘要

马牙门是中国具有较高经济价值的海洋养殖品种之一。本文研究了低温保存(-196℃)对牛颡鱼精子酶活性(SDH、LDH、Na+K+-ATP、SOD、CAT、GR和Acrosin)的影响,以期为牛颡鱼精子低温保存效果提供理论依据,为牛颡鱼人工繁殖奠定基础。我们设置对照组(不加冷冻保护剂)和3个实验组(DMSO(V/V)10%、12.5%、15%),分别于第0、1、3、5、7、15天检测酶活性。结果表明,低温保存后,除GR外,其他酶活性均显著下降(P0.05),其中以对照组下降幅度最大。GR活性在第7天显著升高,对照组显著高于试验组(P0.05),在第15天再次下降。15% DMSO组测定的酶活性高于其他各组,说明15% DMSO对精子酶有保护作用。15天后,15% DMSO溶液条件下,SDH的活动,LDH和Na + / K + atp酶减少从U /毫升(28.500±1.453),(1290.836±27.603)U / L和(2.605±0.232)μ摩尔/ (mg·h)(15.300±0.950)U /毫升(363.713±13.943)U / L和(0.542±0.186)μ摩尔/ (mg·h)分别;SOD和CAT的活动减少从U /毫升(106.497±7.217),(383.632±4.731)U / g(17.036±0.321)U /毫升和(166.940±1.910)/ g分别;精子酵素的活动从(3.521±0.010)减少μ106 IU /μIU / 106(1.212±0.043);但GR的活动从(217.042±6.962)U/L增加到(302.787±24.558)U/L。从下降幅度来看,低温保存对SOD的影响最大,其次是Na+/K+-ATPase。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of cryopreservation on enzyme activity of Exopalaemon carinicauda spermatozoa
Exopalaemon carinicauda is one of the marine aquaculture species with high economic value in China.This paper studied the effects of cryopreservation(-196 ℃)on the enzyme activity(SDH,LDH,Na+K+-ATP,SOD,CAT,GR and Acrosin)in E.carinicauda spermatozoa,in order to provide a theoretical basis for cryopreservation effect on E.carinicauda spermatozoa,and lay a foundation for artificial propagation of E.carinicauda.We set up a control group(without cryoprotectant),and three experimental groups(DMSO(V/V)10%,12.5%,15%),detected the enzyme activities on day 0,1,3,5,7,15.The result showed that,after cryopreservation,in addition to GR,the other enzyme activities decreased significantly(P0.05),with the biggest drop in the control group.GR activity significantly increased in 7 days,and the control group was significantly higher than the experimental group(P0.05),declining again in 15 days.The group of 15% DMSO measured enzyme activities were higher than other groups,indicating that 15% DMSO had protective effect on enzyme of spermatozoa.15 days later,under the condition of 15% DMSO,the activities of SDH,LDH and Na+/K+-ATPase decreased from(28.500±1.453)U/mL,(1290.836±27.603)U/L and(2.605±0.232)μmol/(mg·h)to(15.300±0.950)U/mL,(363.713±13.943)U/L and(0.542±0.186)μmol/(mg·h)respectively;the activities of SOD and CAT decreased from(106.497±7.217)U/mL,(383.632±4.731)U/g to(17.036±0.321)U/mL and(166.940±1.910)U/g respectively;the activities of acrosin decreased from(3.521±0.010)μIU/106 to(1.212±0.043)μIU/106;but the activities of GR increased from(217.042±6.962)U/L to(302.787±24.558)U/L.From the decline range,cryopreservation had the biggest influence on SOD,followed by Na+/K+-ATPase.
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来源期刊
水产学报
水产学报 Environmental Science-Management, Monitoring, Policy and Law
CiteScore
1.40
自引率
0.00%
发文量
5213
期刊介绍: "Fisheries of" mainly reflects the results of scientific research and development of the direction of aquaculture for domestic and foreign academic exchanges Fisheries Service. Mainly basic research published in Fisheries, aquaculture and proliferation of fishing waters environmental protection, preservation of aquatic products processing and utilization, fishing equipment, and other aspects of mechanical papers, research briefings and reviewed.
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