Horizontal and vertical colonies for royal jelly production in Brazil

- The potential of royal jelly production in Africanized honeybee colonies was evaluated using techniques involving two queens per colony in horizontal and vertical systems during the spring of 2019. The techniques were tested for their effect on cell acceptance (%); royal jelly production per colony (g), per harvest (g), and per position of cell bar (g) and compared with standard techniques (five and ten-frame hives) in southern Brazil. Results showed statistical significance in royal jelly production between methods. Vertical colonies produced a significantly greater amount of royal jelly per colony and per harvest (8.26 and 53.28±4.98 g) compared with single-queen colonies in ten-frame hives (4.30 and 32.76±3.57 g) and five-frame hives overlapping (2.03 and 14.45±2.48 g), but did not differ from two-queen horizontal colonies (8.09 and 46.81±4.90 g). In contrast, there was no significant difference in queen cell acceptance rate within vertical, horizontal, and ten-frame colonies. Royal jelly yield of two-queen vertical colonies increase as compared with standard colonies.