O658 Dietzia sp.DQ12-45-1b:O658菌株降解正十六烷代谢产物的检测

Q4 Agricultural and Biological Sciences

应用与环境生物学报 Pub Date : 2013-12-20 DOI:10.3724/SP.J.1145.2013.00519

Xingbiao Wang, Yue-qin Tang, Gang Wu, Xiaolei Wu

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引用次数: 0

摘要

本实验优化了菌株Dietzia sp. DQ12-45-1b生物表面活性剂的提取和检测方法，并研究了菌株在正十六烷上生长不同培养时间的生物表面活性剂产物。乙酸乙酯为最佳提取溶剂，代谢物回收率最高。采用正己烷:氨:异丙醇= 2:1:4 (V:V:V)的混合物作为薄层色谱分析的最佳溶剂体系。菌株DQ12-45-1b产生糖脂类生物表面活性剂，培养液中生物表面活性剂浓度与培养时间呈正相关。乳化十六烷层与水介质中粗提物的含量比为4.16:1。在乳化十六烷层中检测到糖脂，但在水相中未检测到糖脂。图5，表1，文献15

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O658Detection of Metabolites from n -Hexadecane Degradation by Strain Dietzia sp. DQ12-45-1b: O658Detection of Metabolites from n -Hexadecane Degradation by Strain Dietzia sp. DQ12-45-1b

This experiment optimized the extraction and detection methods of biosurfactant produced by the strain Dietzia sp. DQ12-45-1b, and studied biosurfactant products of the strain growing on n-hexadecane for different cultural time. Ethyl acetate was the best extraction solvent with the highest metabolite recovery effi cient. The mixture of hexane : ammonia : isopropanol = 2:1:4 (V:V:V) was used as the best solvent system in thin layer chromatography analysis. The strain DQ12-45-1b produced glycolipid biosurfactant, with the biosurfactant concentration of the culture broth positively correlated with the incubation time. The content ratio of crude extracts in emulsifi ed hexadecane layer and aqueous medium was 4.16:1. A glycolipid was detected in the emulsifi ed hexadecane layer but not in aqueous phase. Fig 5, Tab 1, Ref 15

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来源期刊

应用与环境生物学报 Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

1.20

自引率

0.00%

发文量

4458

期刊介绍： The “Chinese Journal of Applied and Environmental Biology” is a national scientific and technological journal published both at home and abroad. It was started in 1995 and became bimonthly in 1999, and now it is a core journal for applied and environmental biology in China. It aims to introduce and exchange the new achievements, new technologies, new methods and new progresses made from the research fields of biology and its related disciplines both in China and abroad; enhance scientific and technological research serving for the development and construction of national economy, and improvement of people’s living standards.