一株聚羟基烷酸根瘤菌H2-5的筛选与鉴定

Q4 Agricultural and Biological Sciences

应用与环境生物学报 Pub Date : 2013-12-19 DOI:10.3724/SP.J.1145.2013.00375

Yang Shanshan, Zhang Hongrui, Fu Sheng-mi, Li Xinli, C. Jing

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引用次数: 0

摘要

聚羟基烷酸酯(PHA)是由多种细菌合成的一类储碳储能材料。PHA是一种可降解的材料，可用于生物塑料、医用材料等许多领域。本研究从环境中采集了若干土壤或水样，通过富集培养、尼罗蓝荧光染色、苏丹黑染色、傅立叶变换红外光谱元素分析等方法从土壤中分离到一株聚羟基烷酸酯产生菌。根据该菌株的形态特征、生理生化检测和16S rDNA基因序列分析，确定该菌株为Rhizobium sp.H2-5。

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Screening and Identification of a Polyhydroxyalkanoate Producing Strain Rhizobium sp. H2-5

Polyhydroxyalkanoate(PHA) is a family of carbon and energy storage materials synthesized by many bacteria.PHA,as a kind of degradable material,can be used in many areas,such as bioplastics,medical materials etc.In this study,several soil or water samples were collected from environment,and a polyhydroxyalkanoate producing strain was isolated from soil by enrichment culturing,Nile blue fluorescence staining,Sudan black staining,and Fourier transform infrared spectra elemental analysis.This strain was identified and named as Rhizobium sp.H2-5 according to its morphological features,physiological and biochemical tests as well as 16S rDNA gene sequence analysis.

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来源期刊

应用与环境生物学报 Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

1.20

自引率

0.00%

发文量

4458

期刊介绍： The “Chinese Journal of Applied and Environmental Biology” is a national scientific and technological journal published both at home and abroad. It was started in 1995 and became bimonthly in 1999, and now it is a core journal for applied and environmental biology in China. It aims to introduce and exchange the new achievements, new technologies, new methods and new progresses made from the research fields of biology and its related disciplines both in China and abroad; enhance scientific and technological research serving for the development and construction of national economy, and improvement of people’s living standards.