白细胞介素-10（IL-10）感染引起的提取物警告

Medical laboratory technology Pub Date : 2016-08-05 DOI:10.31964/MLTJ.V2I1.43

Denny P.N.H. Margono, E. Suhartono, H. Arwati

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引用次数: 3

摘要

摘要/ Abstract摘要:白细胞介素-10作为促炎细胞因子在重症疟疾损伤中具有保护作用。克拉凯含有具有抗炎活性的生物活性化合物。本研究的目的是确定克拉凯提取物对伯氏单胞杆菌感染小鼠BALB / s白细胞介素-10水平的影响。本研究为纯后测实验研究，采用对照组设计。治疗组分为8组。两组患者分别口服克拉凯提取物10 mg/kgBW和100 mg/kgBW。4组小鼠在感染后3 h及寄生虫率达到15-20%时，分别口服克拉凯提取物10 mg/kgBW和100 mg/kgBW。阴性对照组不接受克拉凯提取物和寄生虫感染。阳性对照组感染寄生虫。治疗4天。最后一次治疗后24小时采集血样。采用夹心ELISA法检测IL-10水平。数据分析采用Games Howell检验，置信水平为95%。治疗组间无显著差异。口服剂量10 mg/KgBW和100 mg/KgBW均未显著提高白细胞介素-10水平。毕业论文关键词:古窄螯虾;摘要:白细胞介素-10 (Interleukin-10)具有促炎作用。克拉凯孟加东森雅瓦生物制剂阳孟加东抗炎药。Tujuan penelitian ini adalah untuk mengetahui potential, ekstrak kelakai terhadap kadar IL-10 pada menencit BALB/s, yang diinfeksi P. berghei ANKA。采用对照组设计，对实验小鼠进行后测。Kelompok perlakuan dibagi menjadi 8 Kelompok。杜阿克龙柏，每口10毫克/公斤巴丹丹和100毫克/公斤巴丹丹。乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木、乌龙木。控制革拉克氏菌和革拉克氏菌阴性。控制白蛉阳性。Perlakuan diberikan selama 4 hari。Sampel darah diambil 24 jam setelah perlakuan terakhir。卡达尔Il-10酶联免疫吸附测定方法。数据分析显示，在游戏中，游戏的成功率为95%。Tidak didapatkan perbedaan bermakna antar kelompok perlakuan。Pemberian Ekstrak kelakai secara口服剂量10 mg/Kg berat badan和100 mg/Kg berat badan显着性脑膜炎katkan kadar白介素-10。Kata-kata Kunci: Stenochlaena palustris(缅甸)Bedd;白细胞介素- 10”

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PENGARUH EKSTRAK KELAKAI (Stenochlaena palustris (Burm.f) Bedd) TERHADAP KADAR INTERLEUKIN-10 (IL-10) MENCIT

Abstract : Interleukin-10 as pro-inflammatory cytokines have a role to protect the damage of severe malaria. Kelakai contain bioactive compounds that have anti-inflammatory activity. The purpose of this study was to determine the potential of the extract kelakai on levels of IL-10 in mice BALB / s infected by P. berghei ANKA. This study is true experimental studies with posttest-only with Control Group Design. The treatment group was divided into 8 groups. Two groups received kelakai extract per oral 10 mg/kgBW and 100 mg/kgBW. Four groups received kelakai extract per oral 10 mg/kgBW and 100 mg/kgBW 3 hours after infection and when the parasitaemia reaches 15-20%. Negative controls do not receive the extract kelakai and parasitic infections. Positive controls get a parasitic infection. Treatment was given for 4 days. Blood samples were taken 24 hours after the last treatment. Levels of IL-10 were measured by sandwich ELISA method. Data were analyzed with Games Howell test, with a confidence level of 95%. There were no significant differences between treatment groups. Extract kelakai Giving oral dose of 10 mg/KgBW and 100 mg/KgBW not significantly increase the levels of interleukin-10. Keywords: Stenochlaena palustris (Burm.f) bedd; Interleukin-10 Abstrak : Interleukin-10 sebagai Sitokin pro-inflamasi memiliki peran melindungi kerusakan pada malaria berat. Kelakai mengandung senyawa bioaktif yang mempunyai aktifitas anti-inflamasi. Tujuan penelitian ini adalah untuk mengetahui potensi ekstrak kelakai terhadap kadar IL-10 pada mencit BALB/s yang diinfeksi P. berghei ANKA. Penelitian ini merupakan studi eksperimental murni dengan Posttest-only with Control Group Design. Kelompok perlakuan dibagi menjadi 8 kelompok. Dua kelompok mendapat ekstrak kelakai per oral 10 mg/kg berat badan dan 100 mg/kg berat badan. Empat kelompok mendapat ekstrak kelakai per oral 10 mg/kg berat badan dan 100 mg/kg berat badan 3 jam setelah infeksi dan pada saat parasitemia mencapai 15-20%. Kontrol negatif tidak mendapat ekstrak kelakai dan infeksi parasit. Kontrol positif mendapat infeksi parasit. Perlakuan diberikan selama 4 hari. Sampel darah diambil 24 jam setelah perlakuan terakhir. Kadar Il-10 diukur dengan ELISA metode sandwich. Data dianalisa dengan tes Games Howell, dengan tingkat kepercayaan 95%. Tidak didapatkan perbedaan bermakna antar kelompok perlakuan. Pemberian Ekstrak kelakai secara oral dosis 10 mg/Kg berat badan dan 100 mg/Kg berat badan tidak signifikan meningkatkan kadar Interleukin-10. Kata-kata Kunci : Stenochlaena palustris (Burm.f) Bedd; Interleukin-10

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Medical laboratory technology

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