脂肪来源间充质干细胞的低温保存。

Chika Miyagi-Shiohira, Kiyoto Kurima, N. Kobayashi, I. Saitoh, Masami Watanabe, Yasufumi Noguchi, Masayuki Matsushita, H. Noguchi
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引用次数: 39

摘要

间充质干细胞(MSCs)具有分化为中胚层细胞的潜力,如成骨细胞、脂肪细胞、肌细胞和软骨细胞。它们具有免疫抑制作用,这使它们成为基于细胞治疗耐药免疫疾病的可行细胞群。脂肪源性间充质干细胞(ASCs)已被证明具有特别容易获得皮下脂肪组织特性的能力,目前冷冻保存是保存ASCs以安全获得大量细胞的常规方法。然而,许多研究报道了冷冻和解冻后的细胞活性可能受到冷冻保存所用溶液的影响。二甲基亚砜(DMSO)通常被用作低温保存介质,因为它通过质膜扩散到细胞中,保护细胞免受冷冻造成的损害。作为DMSO或动物源性血清的替代品,细胞库系列、聚乙烯吡罗烷酮(PVP)、丝胶蛋白和麦芽糖以及甲基纤维素(MC)已被研究用于临床应用。开发一种可靠的细胞冷冻保存方案对于利用间充质干细胞进行再生医学至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cryopreservation of Adipose-Derived Mesenchymal Stem Cells.
Mesenchymal stem cells (MSCs) have the potential to differentiate into cells of mesodermal origin such as osteoblasts, adipocytes, myocytes, and chondrocytes. They possess an immunosuppressive effect, which makes them a viable cell population for the cell-based therapy of treatment-resistant immune diseases. Adipose-derived mesenchymal stem cells (ASCs) have been demonstrated to have the ability to acquire the properties of subcutaneous adipose tissue particularly easily, and cryopreservation is currently performed as a routine method for preserving ASCs to safely acquire large numbers of cells. However, many studies have reported that cellular activity after freezing and thawing may be affected by the solutions used for cryopreservation. Dimethyl sulfoxide (DMSO) is commonly used as a cryopreservation medium as it diffuses into the cell through the plasma membrane and protects the cells from the damage caused by freezing. As substitutes for DMSO or animal-derived serum, cell banker series, polyvinylpyrrolidone (PVP), sericin and maltose, and methyl cellulose (MC) have been investigated for their clinical applications. It is critical to develop a reliable cell cryopreservation protocol for regenerative medicine using MSCs.
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来源期刊
Cell medicine
Cell medicine MEDICINE, RESEARCH & EXPERIMENTAL-
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