人蜕膜间充质细胞是人类诱导多能干细胞的重要来源。

T. Shofuda, D. Kanematsu, H. Fukusumi, A. Yamamoto, Yohei Bamba, S. Yoshitatsu, H. Suemizu, Masato Nakamura, Y. Sugimoto, M. Furue, A. Kohara, W. Akamatsu, Y. Okada, H. Okano, M. Yamasaki, Y. Kanemura
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引用次数: 7

摘要

胎盘组织是一种具有显著再生医学潜力的生物材料。它是由胎儿(羊膜和绒毛膜)和母体(蜕膜)形成的三层结构,含有大量的细胞。此外,收集胎盘组织对供体没有任何身体危险,并且可以与多种HLA类型匹配。蜕膜源性间充质细胞(DMCs)是高度增殖的成纤维细胞样细胞,表达与骨髓源性间充质细胞(BM-MSCs)相似的CD抗原模式。本研究证明,通过逆转录病毒转移重编程因子基因,可以有效地从dmc中产生诱导多能干细胞(iPS)。DMC-hiPS细胞在集落形态、整体基因表达谱(包括人类多能干细胞标记)、OCT3/4和NANOG启动子的DNA甲基化状态以及在体外和体内分化为三种胚层成分的能力方面与人胚胎干细胞(hESCs)具有相当的特征。XIST的RNA表达及其启动子区的甲基化状态表明,DMC-iPSCs在保持未分化和多能性时,有三种不同的状态:(1)完全的x染色体再激活,(2)一条失活的x染色体,或(3)表观遗传畸变。由于dmc来自胎盘的母体部分,因此可以在成年供体完全同意的情况下收集它们,并且对于细胞库和后续一代用于再生应用的人类iPS细胞具有相当大的伦理优势。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Human Decidua-Derived Mesenchymal Cells Are a Promising Source for the Generation and Cell Banking of Human Induced Pluripotent Stem Cells.
Placental tissue is a biomaterial with remarkable potential for use in regenerative medicine. It has a three-layer structure derived from the fetus (amnion and chorion) and the mother (decidua), and it contains huge numbers of cells. Moreover, placental tissue can be collected without any physical danger to the donor and can be matched with a variety of HLA types. The decidua-derived mesenchymal cells (DMCs) are highly proliferative fibroblast-like cells that express a similar pattern of CD antigens as bone marrow-derived mesenchymal cells (BM-MSCs). Here we demonstrated that induced pluripotent stem (iPS) cells could be efficiently generated from DMCs by retroviral transfer of reprogramming factor genes. DMC-hiPS cells showed equivalent characteristics to human embryonic stem cells (hESCs) in colony morphology, global gene expression profile (including human pluripotent stem cell markers), DNA methylation status of the OCT3/4 and NANOG promoters, and ability to differentiate into components of the three germ layers in vitro and in vivo. The RNA expression of XIST and the methylation status of its promoter region suggested that DMC-iPSCs, when maintained undifferentiated and pluripotent, had three distinct states: (1) complete X-chromosome reactivation, (2) one inactive X-chromosome, or (3) an epigenetic aberration. Because DMCs are derived from the maternal portion of the placenta, they can be collected with the full consent of the adult donor and have considerable ethical advantages for cell banking and the subsequent generation of human iPS cells for regenerative applications.
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来源期刊
Cell medicine
Cell medicine MEDICINE, RESEARCH & EXPERIMENTAL-
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