2019-2021年莫斯科临床和食品分离单核细胞增生李斯特菌耐药性分布

Q4 Medicine
S. Ermolaeva, T. Karpova, P. Andriyanov, P. A. Zhurilov, O. Voronina, N. Ryzhova, E. Aksenova, M. Kunda, E. A. Liskova, O. Gruzdeva, E. Klimova, E.A. Posukhovsky, G. Karetkina, A. Melkumyan, O. E. Orlova, E. Burmistrova, T. Pronina, I. Tartakovsky
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引用次数: 0

摘要

目标。目的确定莫斯科与人类李斯特菌病和食品污染相关的单核细胞增生乳杆菌菌株获得性抗微生物药物耐药性的现状。材料与方法。我们使用2019-2021年在莫斯科从临床材料(n = 24)和食品(n = 15)中分离的39株单核增生乳杆菌。采用圆盘扩散法对12种第一、第二防线抗生素的耐药性进行了研究。采用单核增生乳杆菌推荐参数解释结果;结果:所有菌株均对氨苄西林、青霉素、红霉素、万古霉素、亚胺培南、利奈唑胺和阿莫西林/克拉维酸敏感。对庆大霉素耐药(23%),对美罗培南、甲氧苄啶/磺胺甲恶唑和环丙沙星耐药(分别为5%、74%和28%)。共鉴定出15株多重耐药菌株(分别对3种和4种抗生素耐药13株和2株)。9株菌株对甲氧苄啶/磺胺甲恶唑、环丙沙星、左氧氟沙星同时耐药,其中对庆大霉素、甲氧苄啶/磺胺甲恶唑、左氧氟沙星耐药6株,其中对庆大霉素、甲氧苄啶/磺胺甲恶唑、左氧氟沙星耐药3株,对庆大霉素、甲氧苄啶/磺胺甲恶唑、环丙沙星耐药2株。将所研究菌株的氨苄西林和青霉素的生长抑制带与1950-1980年俄罗斯分离的菌株的历史数据进行比较,发现生长抑制带的大小明显向下移动。对比2019 - 2021年不同分离源生长抑制带直径的菌株分布,临床菌株与食品源分离菌株无显著差异。2019-2021年在莫斯科分离的临床和食品来源的单核增生乳杆菌菌株中,获得性耐药性广泛传播。尽管所有菌株都对青霉素敏感,但与1980年以前在俄罗斯分离的单核增生乳杆菌相比,2019-2021年分离的菌株的生长抑制带直径分布明显向氨苄西林和苄西林敏感性降低转变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Distribution of antimicrobial resistance among clinical and food Listeria monocytogenes isolated in Moscow in 2019–2021
Objective. To determine the current state-of-art of acquired resistance to antimicrobial drugs among L. monocytogenes strains associated with listeriosis in humans and food contamination in Moscow. Materials and Methods. We used 39 L. monocytogenes strains isolated in Moscow in 2019–2021 from clinical material (n = 24) and food (n = 15). Resistance to 12 antibiotics of the first and second lines of defense was studied using disk-diffusion method. The parameters recommended for L. monocytogenes were used to interpret the results; in the absence of recommendations for L. monocytogenes, the criteria for Staphylococcus aureus and/or Enterococcus spp. were used. Results. All strains were susceptible to ampicillin, benzylpenicillin, erythromycin, vancomycin, imipenem, linezolid, and the amoxicillin/clavulanic acid. Resistance was observed to gentamicin (23%) as well as to meropenem, trimethoprim/sulfamethoxazole and ciprofloxacin (5%, 74% and 28% of strains, respectively). A total of 15 strains with multiple antibiotic resistance were identified (13 and 2 isolates were resistant to three and four antibiotics, respectively). Simultaneous resistance to trimethoprim/sulfamethoxazole, ciprofloxacin and levofloxacin was observed in 9 strains, 6 strains were resistant to gentamicin and trimethoprim/sulfamethoxazole, including 3 strains – to gentamicin, trimethoprim/sulfamethoxazole, levofloxacin, and 2 strains – to gentamicin, trimethoprim/sulfamethoxazole, ciprofloxacin. Comparison of the growth inhibition zones by ampicillin and benzylpenicillin in the studied strains with historical data on the strains isolated in Russia in 1950–1980 showed a significant downward shift in the size of growth inhibition zones. Comparison of the distribution of strains with different diameters of growth inhibition zones depending on the source of isolation did not show significant differences between clinical strains and strains of food origin isolated in 2019–2021. Conclusions. A wide spread of acquired resistance was shown among L. monocytogenes strains of clinical and food origin isolated in Moscow in 2019–2021. Despite the fact that all strains were susceptible to penicillins, the distribution of growth inhibition zone diameters showed a significant shift towards decreasing sensitivity to ampicillin and benzylpenicillin in strains isolated in 2019–2021 compared with L. monocytogenes strains isolated in Russia before 1980.
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