AtF-box基因表达调控拟南芥根系发育

IF 1 Q3 PLANT SCIENCES
Plant Root Pub Date : 2021-01-01 DOI:10.3117/plantroot.15.69
M. Zhiponova, Jefri Heyman, L. De Veylder, A. Iantcheva
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引用次数: 0

摘要

根系生长受到持续的动态调节,以对发育和环境刺激做出最佳反应。在翻译后水平,蛋白质丰度是由目标蛋白的蛋白酶体降解控制的。这一过程的底物特异性是由参与SCFs (SKP1-CULLIN-F-box蛋白连接酶)E3泛素蛋白连接酶的F-box蛋白发挥的。本研究分析了拟南芥根系发育过程中调节亮氨酸稳态的AtF-box。公开可用的数据集和报告线显示AtF-box在主根和侧根表达。在AtF-box敲低系(AtF-boxamiRNA)的远端干细胞(DSC)中检测到异常的干细胞分裂,这表明AtF-box是最佳细胞分裂所必需的。显微镜观察显示细胞增殖过早退出,细胞分裂活性减慢。相反,AtF-box过表达细胞系(AtFboxOE)细胞分裂期延长。与对照相比,AtF-boxamiRNA和AtF-boxOE的根生长速率分别降低和提高。这些研究结果表明,AtF-box基因在根系生长调控中起着重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
AtF-box gene expression fine-tunes Arabidopsis thaliana root development
Root growth is under constant dynamic regulation for optimal response to developmental and environmental stimuli. At the posttranslational level, protein abundance is controlled by proteasomal degradation of targeted proteins. The substrate-specificity of this process is exerted by F-box proteins taking part in the SCFs (SKP1-CULLIN-F-box protein ligase) E3 ubiquitin protein ligases. In this work an Arabidopsis thaliana AtF-box, which regulates leucine homeostasis, was analyzed in the context of root development. Publicly available data sets and reporter lines revealed AtF-box expression in the primary and lateral roots. Aberrant stem cell divisions were detected in the distal stem cells (DSC) of the AtF-box knockdown lines (AtF-boxamiRNA), suggesting that AtF-box is required for the optimal cell division. Microscopic observations revealed the premature exit from cell proliferation and slower cell division activity. Conversely, in AtF-box overexpression (AtFboxOE) lines the cell division phase was prolonged. The root growth rate was respectively reduced and enhanced in the AtF-boxamiRNA and AtF-boxOE lines compared to the control. From the results of these studies, we concluded that the AtF-box gene is important for the fine-tuning of root growth.
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来源期刊
Plant Root
Plant Root PLANT SCIENCES-
CiteScore
1.50
自引率
0.00%
发文量
2
期刊介绍: Plant Root publishes original papers, either theoretical or experimental, that provide novel insights into plant roots. The Journal’s subjects include, but are not restricted to, anatomy and morphology, cellular and molecular biology, biochemistry, physiology, interactions with soil, mineral nutrients, water, symbionts and pathogens, food culture, together with ecological, genetic and methodological aspects related to plant roots and rhizosphere. Work at any scale, from the molecular to the community level, is welcomed.
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