Varijabilnost i diskriminacijska moć anatomskih parametara kod linija polusrodnika juglans regia podrijetlom iz prirodnih sastojina u Đerdapskoj klisuri, Srbija u rasadničkim uvjetima

This paper presents analysis of anatomical parameters of leaves in Juglans regia half-sib lines from natural stands in Đerdap Gorge in Serbia. Thirty-five half-sib lines were examined, designated according to the number of their mother trees from R1-R35. Twenty measured and derived anatomical parameters were examined, describing stomatal and leaf blade cross-section traits. According to Hierarchical analysis of variance, variation of the majority of characters describing stomatal properties were significantly affected by effect of half-sib lines. The effect of variation between plants within half-sib lines was significant for all examined stomatal traits. However, in cross-section characters, the effect of differences between half-sib lines was significant only for thickness of spongy mesophyll (SL). The discrimination model with seven selected traits achieved 60.5% of correct allocation, while the correct allocation of the model with all 20 examined traits was 81.6%. According to the results of stepwise discriminant analysis, the most powerful trait in discrimination of these half-sib lines were stomatal aperture width/stomatal aperture length ratio (Wb/La) and stomatal aperture length/ stomata guard cell length ratio (La/LA). Presented results strongly support implementation of stomatal and cross-section leaf traits in further studies that would deal with description of variability of Juglans regia.