从南极土壤中分离微藻

Zaima Azira Zainal Abidin, Nurhazrina Zahri, Z. Zainuddin
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引用次数: 0

摘要

微藻是单细胞光合微生物,能够在极端环境条件下生存,特别是低温、高蒸发速率和低水分可用性。来自南极地区的极地微藻因其在极寒环境下的特殊适应性和从各种类型的样品中分离出来的特性而受到研究人员的关注。本研究使用了4个南极土壤样品,分别是样品A、样品B、样品S18和样品S30。所有土壤样品均进行连续稀释并涂在BBM上,然后在25℃周期性光照下孵育3周。经过3周的孵育,只有土壤样品S30微藻呈阳性生长。因此,对土壤样品S30分别采用BBM、3N- BBM+V、JM和MWC培养基进行微藻分离。绿微藻只有在3N-BBM+V和JM培养基中才能获得明显的生长。此外,3N-BBM+V培养基从南极土壤样品中成功回收的微藻菌落数量最多。从南极分离的微藻为各种工业和商业应用提供了资源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ISOLATION OF MICROALGAE FROM ANTARCTIC SOIL
Microalgae are unicellular photosynthetic microorganisms which are able to survive in extreme environmental conditions, specifically low temperature, high evaporation rates as well as low water availability. Polar microalgae from the Antarctic region have received attention from researchers due to their special adaptations for surviving in extremely cold environments and being isolated from various types of samples. Four Antarctic soil samples were used on this study namely-Sample A, Sample B, S18 and S30. All soil samples were subjected to serial dilution and spread on BBM followed by 3 weeks incubation at 25oC with periodic light. Only soil sample S30 showed positive microalgae growth after additional 3 weeks incubation period. Consequently, soil sample S30 was proceeded to isolation of microalgae using BBM, 3N- BBM+V, JM and MWC media. Observable growth of green microalgae could only be obtained using 3N-BBM+V and JM media. Moreover, 3N-BBM+V medium successfully produced the highest number of microalgae colonies recovered from Antarctic soil sample. Isolation of microalgae from Antarctic provides resources for various industrial and commercial applications.
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