蜜蜂体内草甘膦和氨基甲基膦酸的直接测定

Q3 Agricultural and Biological Sciences

Hellenic Plant Protection Journal Pub Date : 2018-01-01 DOI:10.2478/hppj-2018-0005

K. Kasiotis, Z.D. Tzouganaki, K. Machera

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引用次数: 2

摘要

建立了一种简便的LC-ESI-MS/MS方法，用于检测和定量蜜蜂体内除草剂草甘膦(GLY)及其代谢物氨基甲基膦酸(AMPA)。该方法经过验证，符合SANTE 11945/2015指南标准，两种化合物的LOQ和10×LOQ的平均回收率在75-87%之间。测定GLY和AMPA在0.2和0.5 μg/g蜜蜂体重(bw)时的定量限。对14个蜜蜂样本的分析显示，只有一个样本呈阳性，其中含有略高于LOQ的GLY和微量的AMPA。

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Direct determination of glyphosate and aminomethyl phosphonic acid in honeybees

Summary A straightforward LC-ESI-MS/MS method was developed and validated for the detection and quantitation of the herbicide glyphosate (GLY) and its metabolite aminomethyl phosphonic acid (AMPA) in honeybees. The method was validated, fulfilling the SANTE 11945/2015 guideline criteria, demonstrating acceptable mean recoveries at LOQ and 10×LOQ varying from 75-87% for both compounds. LOQ was determined at 0.2 and 0.5 μg/g bee body weight (bw) for GLY and AMPA respectively. Analysis of 14 honeybee samples displayed only one positive sample, containing GLY marginally above LOQ and traces of AMPA.

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来源期刊

Hellenic Plant Protection Journal Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

1.50

自引率

0.00%

发文量