单剂量硒与维生素E对公羊精子发生过程中氧化应激及受精指标的影响

IF 0.6 4区农林科学 Q3 VETERINARY SCIENCES

Acta Veterinaria Brno Pub Date : 2023-01-01 DOI:10.2754/avb202392030259

M. Dolník, D. Mudroňová, G. Lazar, M. Kadáši, Ľ. Horňáková, P. Mudroň

{"title":"单剂量硒与维生素E对公羊精子发生过程中氧化应激及受精指标的影响","authors":"M. Dolník, D. Mudroňová, G. Lazar, M. Kadáši, Ľ. Horňáková, P. Mudroň","doi":"10.2754/avb202392030259","DOIUrl":null,"url":null,"abstract":"The aim of the experiment was to determine the effect of a single subcutaneous administration of selenium (Se) + vitamin E on the ejaculate volume, sperm count and viability, level of apoptosis and oxidative stress (OS) and Se concentration in ejaculates and blood of rams with respect to the time course of the spermatogenic cycle. The experimental group (EG; n = 6) was treated with a single injection of Selevit at a dose of 5 ml per animal (11 mg of sodium selenite and 125 mg of vitamin E/per animal). The control group (CG; n = 4) was treated in the same way, only with saline. Samples of blood and ejaculates were collected from each ram prior to application of Se and on the 1st, 14th, 26th, 38th, 50th and 62nd day after injection. Results showed that the Se concentration in the blood of EG was significantly higher only 24 h after application. Selenium concentration in the ejaculates of the EG was higher during the whole duration of the experiment (62 days), but significantly so only until day 14. The level of OS was significantly reduced on day 1, 14, and 62 after application of Selevit. There were no significant differences in the other analysed indicators. The results showed that one subcutaneous injection of Selevit had a positive effect on Se concentration and OS level in ejaculates, but was not sufficient to improve other monitored sperm quality indicators.","PeriodicalId":7192,"journal":{"name":"Acta Veterinaria Brno","volume":"1 1","pages":""},"PeriodicalIF":0.6000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The effect of a single dose of selenium with vitamin E on oxidative stress and fertilization indicators of ram sperm over the course of spermatogenesis\",\"authors\":\"M. Dolník, D. Mudroňová, G. Lazar, M. Kadáši, Ľ. Horňáková, P. Mudroň\",\"doi\":\"10.2754/avb202392030259\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The aim of the experiment was to determine the effect of a single subcutaneous administration of selenium (Se) + vitamin E on the ejaculate volume, sperm count and viability, level of apoptosis and oxidative stress (OS) and Se concentration in ejaculates and blood of rams with respect to the time course of the spermatogenic cycle. The experimental group (EG; n = 6) was treated with a single injection of Selevit at a dose of 5 ml per animal (11 mg of sodium selenite and 125 mg of vitamin E/per animal). The control group (CG; n = 4) was treated in the same way, only with saline. Samples of blood and ejaculates were collected from each ram prior to application of Se and on the 1st, 14th, 26th, 38th, 50th and 62nd day after injection. Results showed that the Se concentration in the blood of EG was significantly higher only 24 h after application. Selenium concentration in the ejaculates of the EG was higher during the whole duration of the experiment (62 days), but significantly so only until day 14. The level of OS was significantly reduced on day 1, 14, and 62 after application of Selevit. There were no significant differences in the other analysed indicators. The results showed that one subcutaneous injection of Selevit had a positive effect on Se concentration and OS level in ejaculates, but was not sufficient to improve other monitored sperm quality indicators.\",\"PeriodicalId\":7192,\"journal\":{\"name\":\"Acta Veterinaria Brno\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Veterinaria Brno\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.2754/avb202392030259\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Veterinaria Brno","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2754/avb202392030259","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

摘要

本试验旨在探讨单次皮下注射硒(Se) +维生素E对公羊射精量、精子数量和活力、细胞凋亡和氧化应激水平以及精液和血液中硒浓度与生精周期时间的关系。实验组(EG;n = 6)，每只动物单次注射剂量为5ml的Selevit(每只动物11 mg亚硒酸钠和125 mg维生素E)。对照组(CG;N = 4)同样处理，仅用生理盐水。分别于施用硒前和注射后第1、14、26、38、50、62天采集每只公羊的血液和射精标本。结果显示，用药24 h后EG血硒浓度显著升高。在整个试验期间(62 d)，蛋鸡射精中硒的浓度均较高，但仅在试验第14天显著升高。用药后第1、14、62天，OS水平显著降低。其他分析指标无显著差异。结果表明，皮下注射一次Selevit对射精液中硒浓度和OS水平有积极影响，但不足以改善其他监测的精子质量指标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

The effect of a single dose of selenium with vitamin E on oxidative stress and fertilization indicators of ram sperm over the course of spermatogenesis

The aim of the experiment was to determine the effect of a single subcutaneous administration of selenium (Se) + vitamin E on the ejaculate volume, sperm count and viability, level of apoptosis and oxidative stress (OS) and Se concentration in ejaculates and blood of rams with respect to the time course of the spermatogenic cycle. The experimental group (EG; n = 6) was treated with a single injection of Selevit at a dose of 5 ml per animal (11 mg of sodium selenite and 125 mg of vitamin E/per animal). The control group (CG; n = 4) was treated in the same way, only with saline. Samples of blood and ejaculates were collected from each ram prior to application of Se and on the 1st, 14th, 26th, 38th, 50th and 62nd day after injection. Results showed that the Se concentration in the blood of EG was significantly higher only 24 h after application. Selenium concentration in the ejaculates of the EG was higher during the whole duration of the experiment (62 days), but significantly so only until day 14. The level of OS was significantly reduced on day 1, 14, and 62 after application of Selevit. There were no significant differences in the other analysed indicators. The results showed that one subcutaneous injection of Selevit had a positive effect on Se concentration and OS level in ejaculates, but was not sufficient to improve other monitored sperm quality indicators.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Acta Veterinaria Brno 农林科学-兽医学

CiteScore

1.00

自引率

33.30%

发文量

审稿时长

18-36 weeks

期刊介绍： ACTA VETERINARIA BRNO is a scientific journal of the University of Veterinary and Pharmaceutical Sciences in Brno, Czech Republic. The scientific journal Acta Veterinaria Brno is dedicated to the publication of original research findings and clinical observations in veterinary and biomedical sciences. Original scientific research articles reporting new and substantial contribution to veterinary science and original methods that have not been submitted for publication elsewhere are considered for publication. A written statement to this effect should accompany the manuscript, along with approval for publication by the author´s head of department. The authors bear full responsibility for the contents of their contribution. Book reviews are published, too.