熟食和烘焙产品中金黄色葡萄球菌生长和葡萄球菌肠毒素产生的评价

IF 0.6 4区农林科学 Q3 VETERINARY SCIENCES

Acta Veterinaria Brno Pub Date : 2022-01-01 DOI:10.2754/avb202291040417

L. Necidová, Š. Bursová, Danka Haruštiaková, Kateřina Bogdanovičová

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引用次数: 1

摘要

葡萄球菌性食物中毒是世界范围内食源性中毒最常见的原因之一。三明治和甜点易受金黄色葡萄球菌污染，因为在其生产过程中手工操作的比例很高。我们的研究旨在评估储存条件对三明治和奶油泡芙中葡萄球菌肠毒素产生的影响。用能够产生A型、B型和C型葡萄球菌肠毒素的不同金黄色葡萄球菌菌株接种食物，并在15、25和30℃下孵育。在储存期间，对样品进行了金黄色葡萄球菌计数和葡萄球菌肠毒素分析。采用酶联荧光法检测葡萄球菌肠毒素。研究了贮藏不当对金黄色葡萄球菌生长和葡萄球菌肠毒素产生的影响。在任何测试温度下储存72小时的三明治中均未检测到葡萄球菌肠毒素。在奶油泡芙中，在25°C下储存24小时内检测到A、B和C型肠毒素。

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Evaluation of Staphylococcus aureus growth and staphylococcal enterotoxin production in delicatessen and fine bakery products

Staphylococcal food poisoning is one of the most prevalent causes of foodborne intoxication worldwide. Sandwiches and desserts are susceptible to contamination by S. aureus due to the high proportion of manual work during their production. Our study aimed to evaluate the impact of storage conditions on staphylococcal enterotoxin production in sandwiches and buttercream puffs. Foods were inoculated with different S. aureus strains capable of producing type A, B, and C staphylococcal enterotoxins and incubated at 15, 25, and 30 °C. During the storage, samples were analysed for S. aureus counts and for staphylococcal enterotoxins. An enzyme-linked fluorescence assay was used to detect staphylococcal enterotoxins. The influence of inappropriate storage on S. aureus growth and staphylococcal enterotoxin production was evaluated. No staphylococcal enterotoxins were detected in sandwiches stored for 72 h at any of the tested temperatures. In buttercream puffs, enterotoxins type A, B, and C were detected within 24 h of storage at 25 °C.

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来源期刊

Acta Veterinaria Brno 农林科学-兽医学

CiteScore

1.00

自引率

33.30%

发文量

审稿时长

18-36 weeks

期刊介绍： ACTA VETERINARIA BRNO is a scientific journal of the University of Veterinary and Pharmaceutical Sciences in Brno, Czech Republic. The scientific journal Acta Veterinaria Brno is dedicated to the publication of original research findings and clinical observations in veterinary and biomedical sciences. Original scientific research articles reporting new and substantial contribution to veterinary science and original methods that have not been submitted for publication elsewhere are considered for publication. A written statement to this effect should accompany the manuscript, along with approval for publication by the author´s head of department. The authors bear full responsibility for the contents of their contribution. Book reviews are published, too.