纳米银纳米粒甲硝唑-左氧氟沙星复合材料的抗菌及细胞毒活性研究

IF 0.4 Q3 MEDICINE, GENERAL & INTERNAL
O. Ilkov, N. Manko, S. Bilous, G. Didikin, O. Klyuchivska, N. Dilay, R. Stoika
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引用次数: 1

摘要

摘要为研究纳米银粒子电子束固定在甲硝唑和左氧氟沙星晶体上的复合材料的抑菌活性及对人体细胞的毒性作用,采用mtt法评价纳米银粒子甲硝唑和左氧氟沙星复合材料的抑菌活性和细胞毒性。研究对象为金黄色葡萄球菌ATCC25923、大肠杆菌dH5α、铜绿假单胞菌ATCC9027 3株微生物的抗菌活性。用人肾胚HEK 293细胞系研究其细胞毒性作用。对甲硝唑和左氧氟沙星的纳米复合材料进行了抗生素最低毒性剂量浓度和浓度降低/增加2倍的测试。用电子束技术将银纳米粒子固定在甲硝唑和左氧氟沙星表面,对它们的抗菌和细胞毒活性有不同的影响。甲硝唑纳米复合材料对大肠杆菌的抑菌作用弱于单用甲硝唑,而左氧氟沙星纳米复合材料对大肠杆菌的抑菌活性高于单用左氧氟沙星。与游离左氧氟沙星相比,左氧氟沙星纳米复合材料对革兰氏阴性菌(大肠杆菌)的抑菌效果更高,但对铜绿假单胞菌和金黄色葡萄球菌的抑菌活性实际上没有差异。将银纳米颗粒固定在甲硝唑晶体上不影响其对假正常人细胞系HEK 293的细胞毒性,而左氧氟沙星与银纳米复合材料对假正常人细胞系HEK 293的毒性大于单独的左氧氟沙星。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antibacterial and cytotoxic activity of metronidazole and levofloxacin composites with silver nanoparticle
Abstract The aim of the work is to to ascertain their antibacterial activity, as well as the toxic effects toward human cells of composites of silver nanoparticles immobilized by electron-beam technology onto crystals of antimicrobial agents metronidazole and levofloxacin The assessment of antibacterial activity and cytotoxic action of silver naonparticled metronidazole and levofloxacin composites was carried out using the MTT-test. Objects of study of antibacterial activity were three strains of microorganisms: Staphylococcus aureus ATCC25923, Escherichia coli dH5α, Pseudomonas aeruginosa ATCC9027. For the investigation of cytotoxic action, cells of HEK 293 line obtained from human kidney embryos were used. Nanocomposites of metronidazole and levofloxacin were tested at concentrations known as the minimum toxic dose of antibiotics and at concentrations reduced/increased in 2 times. Immobilization of silver nanoparticles on the surface of metronidazole and levofloxacin by electron-beam technology gives a different effect on their antibacterial and cytotoxic activity. Nanocomposites of metronidazole exhibit a weaker antibacterial effect on E. coli than metronidazole alone, while levofloxacin nanocomposites have higher antibacterial activity compared to levofloxacin alone. Nanocomposites of the levofloxacin, compared to free levofloxacin, are characterized by a higher antibacterial effect towards gram-negative bacteria (E. coli), but practically do not differ in activity toward P. aeruginosa and S. aureus. Immobilization of silver nanoparticles on metronidazole crystals does not affect on its cytotoxicity relative to pseudonormal human cells line HEK 293, while the nanocomposites of levofloxacin with silver are more toxic to these cells than levofloxacin alone.
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来源期刊
Current Issues in Pharmacy and Medical Sciences
Current Issues in Pharmacy and Medical Sciences MEDICINE, GENERAL & INTERNAL-
CiteScore
0.80
自引率
0.00%
发文量
28
审稿时长
16 weeks
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