污水中优势肠道病毒的监测为提高急性病毒性肠道感染的流行病学监测效率提供了契机

T. Amvrosieva, N. Paklonskaya, I. Belskaya, Y. Koltunova, Y. Shilova
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引用次数: 0

摘要

背景。监测废水中的肠道病毒是一种新的流行病学方法,可以检测其在人类中的传播强度。该研究的目的是根据实际病原体对白俄罗斯共和国不同地区的急性病毒性肠道感染(AEI)患者的废水和生物材料进行平行监测研究并进行分析。材料和方法。采用实时聚合酶链反应(real-time PCR)对白俄罗斯不同地区收集的403份AEI患者废水和381份患者粪便进行检测。结果。在AEI患者中,轮状病毒A(20.4%)和诺如病毒2基因组(10.2%)最常被检测到,而腺病毒F(2.2%)、肠病毒(0.8%)和诺如病毒1(0.3%)则很少被发现。腺病毒F(25.9%)、轮状病毒A(18.4%)和肠病毒(13.4%)在废水样品中占主导地位,而诺如病毒2(6.8%)和诺如病毒1的检出率较低(1.5%)。在患者体内和废水中发现的病毒AСI病原体的百分比显示出一定的差异,这表明其中一些(腺病毒F和肠病毒)存在活跃的隐性循环。在其中一份废水样本中检测到SARS-CoV-2冠状病毒。经测序和生物信息学分析,其核苷酸序列与covid -19患者分离株序列相似度为100%。所获得的数据表明,基于废水中肠道病毒监测的研究具有潜力,可以提高对已知AEI病原体循环的流行病学监测的有效性,并发现新的和正在出现的病原体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
THE MONITORING OF DOMINANT ENTERIC VIRUSES IN WASTEWATER AS AN OPPORTUNITY TO IMPROVE THE EFFICIENCY OF EPIDEMIOLOGICAL SURVEILLANCE OF ACUTE VIRAL INTESTINAL INFECTIONS
Background. The monitoring of enteric viruses in wastewater is a new epidemiological approach allowing to detect the intensity of their circulation in humans. The aim of the study is to conduct and analyze parallel monitoring studies of wastewater and biological material from patients with acute viral intestinal infections (AEI) selected from different regions of the Republic of Belarus in terms of their actual pathogens. Material and methods. 403 samples of wastewater and 381 samples of feces from patients with AEI, collected in different regions of Belarus, were examined by real-time PCR. Results. In patients with AEI, rotaviruses A (20,4%) and noroviruses of the genogroup 2 (10,2%) were most often detected, while adenoviruses F (2,2%), enteroviruses (0,8%) and noroviruses 1 (0,3%) were found quite rarely. Adenoviruses F (25,9%), rotaviruses A (18,4%) and enteroviruses (13,4%) dominated in wastewater samples while noroviruses 2 (6,8%) and noroviruses 1 were detected much less frequently (1,5%). Certain differences were revealed in the percentage of viral AСI pathogens present in patients and those found in wastewater, that indicates active hidden circulation of some of them (adenoviruses F and enteroviruses). SARS-CoV-2 coronavirus was detected in one of the wastewater samples. The conducted sequencing and bioinformatic analysis of its nucleotide sequence showed 100% similarity with the sequences of isolates identifed in patients with COVID-19.Conclusions. The obtained data indicate the potential of the studies based on monitoring of intestinal viruses in wastewater in order to increase the effectiveness of epidemiological surveillance of known AEI pathogens circulation and to identify new and emerging ones.
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