红光(660 nm)对体外金黄色葡萄球菌在亚甲蓝存在下的光灭活作用

У Присутності, Метиленового Синього, П.А. Вірич, О.М. Надтока, Наталiя Куцевол, Богдан Криса
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引用次数: 1

摘要

介绍。Оpen伤口和溃疡的治疗包括使用绷带材料、抗生素和防腐剂,以防止致病性微生物群的发展,并为组织再生提供必要的条件。多重耐药菌株的出现降低了这种技术的有效性,需要新的治疗方法。其中一个有前途的领域是使用外部光敏剂的光动力疗法。本研究的目的是确定红光(660 nm)和不同浓度亚甲基蓝协同作用对金黄色葡萄球菌生长的抑制效果。材料和方法。以右旋糖酐-聚丙烯酰胺共聚物为基础,在交联剂浓度为0.2%、0.4%、0.6% (w/w)的情况下制备水凝胶,考察了亚甲基蓝在水凝胶中的扩散速率。对金黄色葡萄球菌野生菌株在蛋黄盐琼脂上进行了微生物学研究。在 ller- hinton 2号琼脂上评价亚甲基蓝的杀菌作用,类似于评估微生物对抗生素耐药性的圆盘扩散法。对于不同波长的照射,使用«LIKA-Led»(Photonics Plus) led 390 nm,460 nm和660 nm。每个波长的辐射功率为100 mW,持续时间为- 20 min、30 min、40 min,照射剂量根据持续时间分别为21 J/cm2、31.5 J/cm2、42.1 J/cm2。在OriginLab 8.0软件包中对数据进行数理统计处理。在基于葡聚糖-聚丙烯酰胺共聚物的水凝胶中增加交联剂的量可以降低亚甲基蓝从水凝胶中的扩散速率。390 nm紫外线照射20分钟可使金黄色葡萄球菌菌落数量减少80%。暴露量的进一步增加并没有导致该指标的显著变化。蓝光(460 nm)在20分钟的曝光下可将该菌株的存在减少66%,并在30分钟的曝光下达到紫外线的效果。红光(660 nm)无杀菌作用。在浓度为0.001%和0.0001%时,亚甲蓝的活性最小,约为6毫米。0.001%亚甲基蓝与红光协同作用可使活性提高40%,达10 mm。对于金黄色葡萄球菌的光灭活,建议使用660 nm波长的低能量红光与饱和亚甲基蓝(0.001%)水凝胶结合使用。也许红光和染料的协同作用提供了一代活性自由基,有助于微生物的生长。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Photoinactivation of staphylococcus aureus in vitro by red light (660 nm) in the presence of methylene blue
Introduction. Оpen wounds and ulcers treatment involves the use of bandage material, antibiotics and antiseptic to prevent the development of a pathogenic microflora and to provide the necessary conditions for tissue regeneration. An emergence of multi-resistant strains of microorganisms reduces the effectiveness of such technology and requires the new treatment approaches. One of the promising areas is a photodynamic therapy with the use of external photosensitizers. The aim of the investigation is to determine the effectiveness of the synergistic action of red light (660 nm) and different concentrations of methylene blue on the inhibition of Staphylococcus aureus growth. Materials and methods. We used the hydrogels based on the copolymers dextran-polyacrylamide with the different concentration of crosslinking agent 0.2 %, 0.4 %, 0.6 % (w/w) for investigation a rate of diffusion methylene blue into and out from hydrogel. Microbiological research was performed on wild strains of S. aureus isolated on a Yolk-salt agar. The evaluation of a bactericidal action of methylene blue was carried out on a Müller-Hinton No. 2 agar similarly to the disc-diffusion method for assessing the resistance of microorganisms to antibiotics. For irradiation by different wavelengths was used «LIKA-Led» (Photonics Plus) LEDs 390 nm, 460 nm and 660 nm. The radiation power for each wavelength was 100 mW, duration — 20 min, 30 min, 40 min. According to the duration, the irradiation doses were 21 J/cm2, 31.5 J/cm2, 42.1 J/cm2. Mathematical and statistical data processing was performed in the OriginLab 8.0 software package. Results. Increasing the amount of crosslinking agent in the hydrogel based on the copolymer dextran-polyacrylamide provides a decrease in the diffusion rate of methylene blue from the hydrogel. 390 nm ultraviolet radiation reduces the number of S. aureus colonies for 80% at 20 min exposure. Further increase in the exposure did not contribute to significant changes in this indicator. Blue light (460 nm) reduces the presence of this strain of microorganisms for 66% at 20 min exposure and reaches the effect of UV at 30 min exposure. Red light (660 nm) has no bactericidal effect. Minimal activity was found for methylene blue at concentrations of 0.001% and 0.0001% which was around 6 mm. The synergistic effect of 0.001% methylene blue and red light increases the activity for 40% up to 10 mm. Conclusions. For the photoinactivation of Staphylococcus aureus, it is advisable to use a low energy red light with a 660 nm wavelength in combination with a saturated methylene blue (0.001%) hydrogels. Perhaps the synergistic action of red light and dye provides a generation of active radicals that contribute to the growth retardation of microorganisms.
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