从紫荆叶提取物中分离出两个具有抗疟活性的同源三萜

Q3 Pharmacology, Toxicology and Pharmaceutics

ACTA Pharmaceutica Sciencia Pub Date : 2019-01-01 DOI:10.23893/1307-2080.APS.05715

Wande M Oluyemi, B. Samuel, Kahlig Hans-peter, Taramelli Donatella, Krenn Liselotte

{"title":"从紫荆叶提取物中分离出两个具有抗疟活性的同源三萜","authors":"Wande M Oluyemi, B. Samuel, Kahlig Hans-peter, Taramelli Donatella, Krenn Liselotte","doi":"10.23893/1307-2080.APS.05715","DOIUrl":null,"url":null,"abstract":"Combretum genus (Combretaceae) is used locally in the treatment of various diseases including malaria. Current study aimed to investigate the antiplasmodial activities of methanol extract of C. zenkeri in Plasmodium falciparum chloroquine sensitive (D10) and resistant (W2) strains and identify the bioactive principles. Repeated chromatographic separation was carried out on the chloroform fraction to afford the isolation of bioactive compounds which was characterized by application of spectroscopic techniques (ESI-MS, HR-ESIMS, 1D and 2D NMR). Antiplasmodial activities of chloroform fraction (D10; IC50= 12.57±1.57 μg/mL and W2; IC50= 12.14±0.95 μg/mL) showed more activity than the n-butanol fraction (D10; IC50= 61.98±3.25 μg/mL and W2; IC50= 61.26±8.64 μg/mL). Phytochemical investigation of the extract afforded isolation of two triterpenes with antimalarial activities. This paper identified for the first time the antimalarial principles in C. zenkeri as ursolic and oleanolic acids; which justified the local use of the plant in the treatment of malaria.","PeriodicalId":6962,"journal":{"name":"ACTA Pharmaceutica Sciencia","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Isolation of two homologous triterpenes with antimalarial activities from the leaf extract of combretum zenkeri\",\"authors\":\"Wande M Oluyemi, B. Samuel, Kahlig Hans-peter, Taramelli Donatella, Krenn Liselotte\",\"doi\":\"10.23893/1307-2080.APS.05715\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Combretum genus (Combretaceae) is used locally in the treatment of various diseases including malaria. Current study aimed to investigate the antiplasmodial activities of methanol extract of C. zenkeri in Plasmodium falciparum chloroquine sensitive (D10) and resistant (W2) strains and identify the bioactive principles. Repeated chromatographic separation was carried out on the chloroform fraction to afford the isolation of bioactive compounds which was characterized by application of spectroscopic techniques (ESI-MS, HR-ESIMS, 1D and 2D NMR). Antiplasmodial activities of chloroform fraction (D10; IC50= 12.57±1.57 μg/mL and W2; IC50= 12.14±0.95 μg/mL) showed more activity than the n-butanol fraction (D10; IC50= 61.98±3.25 μg/mL and W2; IC50= 61.26±8.64 μg/mL). Phytochemical investigation of the extract afforded isolation of two triterpenes with antimalarial activities. This paper identified for the first time the antimalarial principles in C. zenkeri as ursolic and oleanolic acids; which justified the local use of the plant in the treatment of malaria.\",\"PeriodicalId\":6962,\"journal\":{\"name\":\"ACTA Pharmaceutica Sciencia\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACTA Pharmaceutica Sciencia\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.23893/1307-2080.APS.05715\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Pharmacology, Toxicology and Pharmaceutics\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACTA Pharmaceutica Sciencia","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.23893/1307-2080.APS.05715","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}

引用次数: 0

摘要

Combretum属(combreaceae)在当地用于治疗包括疟疾在内的各种疾病。本研究旨在研究金针叶甲醇提取物对恶性疟原虫氯喹敏感(D10)和耐药(W2)菌株的抗疟原虫活性，并确定其生物活性原理。对氯仿部分进行重复色谱分离，分离出生物活性化合物，并应用波谱技术(ESI-MS、HR-ESIMS、1D和2D NMR)对其进行了表征。氯仿组分(D10)的抗疟原虫活性;IC50= 12.57±1.57 μg/mL, W2;IC50= 12.14±0.95 μg/mL)的活性高于正丁醇部分(D10;IC50= 61.98±3.25 μg/mL, W2;IC50= 61.26±8.64 μg/mL)。对提取物进行植物化学研究，分离出两种具有抗疟活性的三萜。本文首次鉴定出仙子的抗疟作用机理为熊果酸和齐墩果酸;这证明了在当地使用这种植物治疗疟疾是合理的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Isolation of two homologous triterpenes with antimalarial activities from the leaf extract of combretum zenkeri

Combretum genus (Combretaceae) is used locally in the treatment of various diseases including malaria. Current study aimed to investigate the antiplasmodial activities of methanol extract of C. zenkeri in Plasmodium falciparum chloroquine sensitive (D10) and resistant (W2) strains and identify the bioactive principles. Repeated chromatographic separation was carried out on the chloroform fraction to afford the isolation of bioactive compounds which was characterized by application of spectroscopic techniques (ESI-MS, HR-ESIMS, 1D and 2D NMR). Antiplasmodial activities of chloroform fraction (D10; IC50= 12.57±1.57 μg/mL and W2; IC50= 12.14±0.95 μg/mL) showed more activity than the n-butanol fraction (D10; IC50= 61.98±3.25 μg/mL and W2; IC50= 61.26±8.64 μg/mL). Phytochemical investigation of the extract afforded isolation of two triterpenes with antimalarial activities. This paper identified for the first time the antimalarial principles in C. zenkeri as ursolic and oleanolic acids; which justified the local use of the plant in the treatment of malaria.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

ACTA Pharmaceutica Sciencia Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

0.90

自引率

0.00%

发文量

期刊介绍： Acta Pharmaceutica Sciencia (Acta Pharm. Sci.), formerly known as Bulletin of Pharmacy and Acta Pharmaceutica Turcica is a peer-reviewed scientific journal publishing current research and reviews covering all fields of pharmaceutical sciences since 1953. The original studies accepted for publication must be unpublished work and should contain data that have not been published elsewhere as a whole or a part. The reviews must provide critical evaluation of the state of knowledge related with the subject. All manuscripts has to be written in clear and concise English. Starting from 2016, the journal will be issued quarterly both in paper and on-line formates also publish special issues for national or international scientific meetings and activities in the coverage field.