DNA指纹分析在西伯利亚鲟混合种群鉴定中的应用

Q4 Agricultural and Biological Sciences

Polish Journal of Natural Sciences Pub Date : 2009-09-01 DOI:10.2478/V10020-009-0016-8

D. Fopp‐Bayat

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引用次数: 6

摘要

应用微卫星DNA指纹图谱技术对商业生产的西伯利亚鲟混合雌化子代和个体进行了分离。对母系亲本的11个微卫星DNA位点进行了变异分析。从而获得了所研究位点的微卫星DNA图谱，并将这一关键点应用于混合鱼的分离分析。结果281个个体中有108个被证实为雌性发生后代。目前的微卫星变异调查证明了一种可靠的分离混合鱼群的工具。

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APPLICATION DNA FINGERPRINT ANALYSIS FOR IDENTIFICATION OF MIXED GROUPS OF SIBERIAN STURGEON (ACIPENSER BAERI BRANDT)

DNA finferprinting analysis based on microsatellites was applied for separation of mixed gynogenetic offspring of Siberian sturgeon (Acipenser baeri) and individuals from commercial production. Variation at 11 microsatellite DNA loci was surveyed for parent of gynogenetic offspring. Thus microsatellite DNA profiles in studied loci were known and this key-point was aplied in segregation analysis of mixed fish. In results 108 individuals of 281 studied were verified as gynogenetic offspring. The present survey of microsatellite variation demonstrated a reliable tool for separation of mixed group of fish.

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来源期刊

Polish Journal of Natural Sciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

0.60

自引率

0.00%

发文量