番木瓜另一姊妹枝巧克力辣椒的核型和基因组大小的测定

Q3 Agricultural and Biological Sciences

Plant Omics Pub Date : 2021-01-01 DOI:10.21475/poj.14.01.21.p2944

Dessireé Zerpa‑Catanho, T. Jatt, R. Ming

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引用次数: 0

摘要

辣椒是一种草本植物，属于辣椒属和加勒比科。目前还没有关于巧克力豆染色体数目或基因组大小的信息来证实异倍体事件的发生和探索异型性染色体的存在。因此，通过核型和观察到的染色体数的计数来确定该物种的染色体总数，并通过流式细胞术分别估计雌雄植株的基因组大小。结果表明，J. chocola有8对染色体(2n = 2x = 16)，其中5对染色体为顺心染色体，2对为亚顺心染色体，1对为远心染色体。以Phaseolus vulgaris (1C = 0.60 pg)和Carica papaya (1C = 0.325 pg)两种植物为标准，分别估算雌雄植株picograms核DNA含量(1C值)和二倍体基因组大小，寻找异型性染色体存在的可能性。木瓜被证明是测定巧克力螟DNA含量和二倍体基因组大小的较好标准。雌株(1C = 1.02±0.003 pg)与雄株(1C = 1.02±0.008 pg) DNA含量无显著差异。根据得到的c值计算出巧克力j.s choola单倍体基因组碱基对的估计基因组大小。结果表明，雌雄植株单倍体基因组大小分别为1018.44±3.07 Mb和1022.08±7.76 Mb。根据观察到的染色体数目和基因组大小，在贾里拉地区只发生了两次报道的异倍体事件中的一次，没有发现异型性染色体的证据。这些结果提供了可可树基因组的基本信息，并将加快对该物种，贾里拉属和加勒比科的性染色体和基因组进化的研究

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Karyotype and genome size determination of Jarilla chocola, an additional sister clade of Carica papaya

Jarilla chocola is an herbaceous plant species that belongs to the Jarilla genus and the Caricaceae family. No information on chromosome number or genome size has been reported for J. chocola that confirms the occurrence of dysploidy events and explore the existence of heteromorphic sex chromosomes. Therefore, the total number of chromosomes of this species was determined by karyotyping and counting the number of chromosomes observed, and the genome size of female and male plants was estimated separately by flow cytometry. Results showed that J. chocola has eight pairs of chromosomes (2n = 2x = 16), and its chromosomes are classified as metacentric for five pairs, submetacentric for two pairs and telocentric for one pair. The nuclear DNA content (1C-value) in picograms and diploid genome size was estimated separately from female and male plants using two species as the standards, Phaseolus vulgaris (1C = 0.60 pg) and Carica papaya (1C = 0.325 pg), to look for the possible existence of heteromorphic sex chromosomes. C. papaya proved to be a better standard for the determination of J. chocola DNA content and diploid genome size. No significant difference on the DNA content was observed between female (1C = 1.02 ± 0.003 pg) and male (1C = 1.02 ± 0.008 pg) plants. The estimated genome size of J. chocola per haploid genome in base pairs was calculated from the obtained C-values. Results showed an estimated genome size per haploid genome of 1018.44 ± 3.07 Mb and 1022.08 ± 7.76 Mb for female and male plants, respectively. Due to the observed chromosome number and genome size, only the occurrence of one of two previously reported dysploidy events in Jarilla could be confirmed for J. chocola and no evidence of heteromorphic sex chromosomes was found. These results provide fundamental information of the J. chocola genome and will expedite investigation of sex chromosomes and genome evolution in this species, the Jarilla genus and the Caricaceae family

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来源期刊

Plant Omics 生物-植物科学

CiteScore

1.30

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Plant OMICS is an international, peer-reviewed publication that gathers and disseminates fundamental and applied knowledge in almost all area of molecular plant and animal biology, particularly OMICS-es including: Coverage extends to the most corners of plant and animal biology, including molecular biology, genetics, functional and non-functional molecular breeding and physiology, developmental biology, and new technologies such as vaccines. This journal also covers the combination of many areas of molecular plant and animal biology. Plant Omics is also exteremely interested in molecular aspects of stress biology in plants and animals, including molecular physiology.