以枯草芽孢杆菌ch -13为基础的生物杀菌剂处理蘑菇青霉病的工艺改进

I. Potocnik, J. Luković, B. Todorovic, M. Stepanović, L. Santric, S. Milijasevic-Marcic, E. Rekanović
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引用次数: 0

摘要

先前的研究证实了枯草芽孢杆菌Ch-13生物杀菌剂在防治绿霉病和提高双孢蘑菇产量方面具有良好的选择性。推荐用量为3次(30 + 15 + 15 ml m-2),蘑菇产量提高8.41%,对绿霉病(侵袭性木霉)防治效果提高53.57%。然后进一步研究了生物杀菌剂枯草芽孢杆菌Ch-13的不同应用程序,涉及6次拆分剂量(6 ?10毫升m-2)的总剂量相同的60毫升,这是以前测试的三个分剂量。在实验菌室(体内)对人工侵染欧洲木霉的白针菇进行了生物杀菌剂对子实体产量的影响及防治效果的评价。杀菌剂丙氯嗪(71.08%)与生物杀菌剂6次(63.05%)、3次(58.43%)的防治效果差异无统计学意义。杀菌剂/生物杀菌剂对食用菌产量的正向影响,各处理间差异无统计学意义。基于枯草芽孢杆菌ch -13的生物杀菌剂可根据蘑菇栽培期间的浇水或采摘时间,分3次或6次施用,两种方案均具有满意的防病效果和对蘑菇产量的积极影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Improvement of procedure for casing treatment with a bacillus subtilis Ch-13-based biofungicide to control green mould disease of mushrooms
A previous study had confirmed good selective features of a biofungicide based on Bacillus subtilis Ch-13 regarding green mould disease control and increase in white button mushroom (Agaricus bisporus) production. The recommended application rate of the biofungicide in three split doses (30 + 15 + 15 ml m-2) enhanced mushroom yield by 8.41% and control of green mould disease (Trichoderma aggressivum) by 53.57%. A different application procedure of the biofungicide B. subtilis Ch-13 was then further investigated, involving six split doses (6 ? 10 ml m-2) of the same total dose of 60 ml which was previously tested in three split doses. The impact of the biofungicide on fruiting body yield and its effectiveness in disease control were evaluated on white button mushroom artificially infected with Trichoderma aggressivum f. europaeum in the experimental mushroom growing room (in vivo). No statistically significant differences in disease control efficacy were found between the fungicide prochloraz (71.08%) and the biofungicide applied either in six split doses (63.05%) or three (58.43%). As for the fungicide/biofungicide positive impact on mushroom production, no statistically significant differences were detected among treatments. The B. subtilis Ch-13-based biofungicide may be applied in three split doses, as well as in six split applications, depending on the watering or picking schedule during mushroom cultivation, as both schemes showed satisfactory efficacy in disease control and positive effects on mushroom yield.
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