{"title":"施用有机纳米肥料后豌豆花粉萌发及活力的研究","authors":"N. Georgieva, I. Nikolova, V. Kosev, Y. Naydenova","doi":"10.2298/PIF1701061G","DOIUrl":null,"url":null,"abstract":"The objective of this study was to evaluate the influence of two organic nanofertilizers, Lithovit and Nagro, on in vitro germination, pollen tube elongation and pollen grain viability of Pisum sativum L cv. Pleven 4. The effect of their application was high and exceeded data for the untreated control (44.2 and 47.23 % regarding pollen germination and pollen tube elongation, respectively), as well as the effect of the control organic algal fertilizer Biofa (17.5 and 27.9 %, respectively). Pollen grains were inoculated in four culture media. A medium containing 15% sucrose and 1% agar had the most stimulating impact on pea pollen grains. Pollen viability, evaluated by staining with 1% carmine, was within limits of 74.72-87.97%. The highest viability of pollen grains was demonstrated after the application of Nagro organic nano-fertlizer.","PeriodicalId":19801,"journal":{"name":"Pesticidi I Fitomedicina","volume":"74 1","pages":"61-65"},"PeriodicalIF":0.0000,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"6","resultStr":"{\"title\":\"In vitro germination and viability of pea pollen grains after application of organic nano-fertilizers\",\"authors\":\"N. Georgieva, I. Nikolova, V. Kosev, Y. Naydenova\",\"doi\":\"10.2298/PIF1701061G\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The objective of this study was to evaluate the influence of two organic nanofertilizers, Lithovit and Nagro, on in vitro germination, pollen tube elongation and pollen grain viability of Pisum sativum L cv. Pleven 4. The effect of their application was high and exceeded data for the untreated control (44.2 and 47.23 % regarding pollen germination and pollen tube elongation, respectively), as well as the effect of the control organic algal fertilizer Biofa (17.5 and 27.9 %, respectively). Pollen grains were inoculated in four culture media. A medium containing 15% sucrose and 1% agar had the most stimulating impact on pea pollen grains. Pollen viability, evaluated by staining with 1% carmine, was within limits of 74.72-87.97%. The highest viability of pollen grains was demonstrated after the application of Nagro organic nano-fertlizer.\",\"PeriodicalId\":19801,\"journal\":{\"name\":\"Pesticidi I Fitomedicina\",\"volume\":\"74 1\",\"pages\":\"61-65\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pesticidi I Fitomedicina\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2298/PIF1701061G\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pesticidi I Fitomedicina","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2298/PIF1701061G","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 6
摘要
本试验旨在研究两种有机纳米肥料Lithovit和Nagro对豌豆(Pisum sativum L cv)离体萌发、花粉管伸长和花粉粒活力的影响。普列文4。施用这两种有机肥的效果均高于对照(花粉萌发率和花粉管伸长率分别为44.2%和47.23%)和对照有机藻肥Biofa(分别为17.5%和27.9%)。将花粉粒接种于4种培养基中。含有15%蔗糖和1%琼脂的培养基对豌豆花粉粒的刺激作用最大。1%胭脂红染色评价花粉活力在74.72 ~ 87.97%范围内。施用纳格罗有机纳米肥料后，花粉粒活力最高。
In vitro germination and viability of pea pollen grains after application of organic nano-fertilizers
The objective of this study was to evaluate the influence of two organic nanofertilizers, Lithovit and Nagro, on in vitro germination, pollen tube elongation and pollen grain viability of Pisum sativum L cv. Pleven 4. The effect of their application was high and exceeded data for the untreated control (44.2 and 47.23 % regarding pollen germination and pollen tube elongation, respectively), as well as the effect of the control organic algal fertilizer Biofa (17.5 and 27.9 %, respectively). Pollen grains were inoculated in four culture media. A medium containing 15% sucrose and 1% agar had the most stimulating impact on pea pollen grains. Pollen viability, evaluated by staining with 1% carmine, was within limits of 74.72-87.97%. The highest viability of pollen grains was demonstrated after the application of Nagro organic nano-fertlizer.
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