ASSESSMENT OF GENETIC DIVERSITY IN YEAST AND BARLEY BY RETROTRANSPOSON-BASED MOLECULAR MARKERS

Five Molecular genetics techniques (RAPD, ISSRs, IRAP, REMAP and RRAP) were used to study the genetic diversity in five yeast strains and six barley cultivars. New two retrotransposon-based primers (ScM1 and ScM2) were designed. RAPD technique represented the highest polymorphism percentages per primer compared with the other four techniques. ISSR primers represented polymorphism percentages more than IRAP primers in yeast, but lower in barley. Both REMAP and RRAP combinations showed similar results either in yeast or barley. Retrotransposon-based techniques (IRAP, RAP and REMAP) showed more number of bands more than those non-retrotransposon (RAPD and ISSRs) based techniques which make them a useful approach as molecular markers.