利用SSR引物对水稻不同亚种进行区分

A. Fayed, N. Abbas
{"title":"利用SSR引物对水稻不同亚种进行区分","authors":"A. Fayed, N. Abbas","doi":"10.21608/EJGC.2014.9938","DOIUrl":null,"url":null,"abstract":"Nine rice (Oryza sativa L.) genotypes of indica and japonica types were used in the present study. Total number of 274 SSR simple sequence repeats primers were used to detect the genetic variation and genetic markers specific for each rice genotype. The results showed that the 219 SSR primers were generate a total 645 amplified alleles, among them 605 alleles were polymorphic. The temperate japonica accessions showed 105 alleles at 50 polymorphic loci. Within three indica accessions, 266 alleles were detected at 122 polymorphic loci. Within the three tropical japonica accessions, 129 alleles were detected at 61 polymorphic loci, respectively. The average number of alleles per locus (A) 2.49, 1.2, 1.55 and 1.25 were observed in total, temperate japonica, indica, and tropical japonica accessions, respectively. Average number of alleles per polymorphic locus (Ap) 2.76, 2.1, 2.18 and 2.11 were observed in total, temperate japonica, indica, and tropical japonica accessions, respectively. The average number of gene diversity (PIC) was 0.51, indicating moderately high variation among the nine accessions. Twelve SSR markers were able to discriminate among the temperate japonica, indica, and tropical japonica rice accessions under the present study.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"43 1","pages":"157-171"},"PeriodicalIF":0.0000,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"UTILIZATION OF SSR PRIMERS TO DISCRIMINATE BETWEEN DIFFERENT SUBSPECIES OF RICE (Oryza sativa, L.)\",\"authors\":\"A. Fayed, N. Abbas\",\"doi\":\"10.21608/EJGC.2014.9938\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Nine rice (Oryza sativa L.) genotypes of indica and japonica types were used in the present study. Total number of 274 SSR simple sequence repeats primers were used to detect the genetic variation and genetic markers specific for each rice genotype. The results showed that the 219 SSR primers were generate a total 645 amplified alleles, among them 605 alleles were polymorphic. The temperate japonica accessions showed 105 alleles at 50 polymorphic loci. Within three indica accessions, 266 alleles were detected at 122 polymorphic loci. Within the three tropical japonica accessions, 129 alleles were detected at 61 polymorphic loci, respectively. The average number of alleles per locus (A) 2.49, 1.2, 1.55 and 1.25 were observed in total, temperate japonica, indica, and tropical japonica accessions, respectively. Average number of alleles per polymorphic locus (Ap) 2.76, 2.1, 2.18 and 2.11 were observed in total, temperate japonica, indica, and tropical japonica accessions, respectively. The average number of gene diversity (PIC) was 0.51, indicating moderately high variation among the nine accessions. Twelve SSR markers were able to discriminate among the temperate japonica, indica, and tropical japonica rice accessions under the present study.\",\"PeriodicalId\":31811,\"journal\":{\"name\":\"Egyptian Journal of Genetics and Cytology\",\"volume\":\"43 1\",\"pages\":\"157-171\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2014-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Egyptian Journal of Genetics and Cytology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21608/EJGC.2014.9938\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Egyptian Journal of Genetics and Cytology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/EJGC.2014.9938","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

本研究采用籼稻和粳稻九种水稻基因型。利用274个SSR简单序列重复引物对各水稻基因型的遗传变异和特异遗传标记进行检测。结果表明,219个SSR引物共扩增出645个等位基因,其中605个等位基因为多态。温带粳稻材料在50个多态位点上有105个等位基因。在3份籼稻材料中,共检测到266个等位基因和122个多态性位点。在3份热带粳稻材料中,分别在61个多态性位点检测到129个等位基因。平均等位基因数(A)分别为2.49、1.2、1.55和1.25,温带粳稻、籼稻和热带粳稻的等位基因数分别为2.49、1.2、1.55和1.25。总粳、温带粳、籼稻和热带粳的平均等位基因数分别为2.76、2.1、2.18和2.11。9份材料的平均基因多样性(PIC)为0.51,表明9份材料间存在中等程度的变异。在本研究中,有12个SSR标记能够对温带粳稻、籼稻和热带粳稻材料进行区分。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
UTILIZATION OF SSR PRIMERS TO DISCRIMINATE BETWEEN DIFFERENT SUBSPECIES OF RICE (Oryza sativa, L.)
Nine rice (Oryza sativa L.) genotypes of indica and japonica types were used in the present study. Total number of 274 SSR simple sequence repeats primers were used to detect the genetic variation and genetic markers specific for each rice genotype. The results showed that the 219 SSR primers were generate a total 645 amplified alleles, among them 605 alleles were polymorphic. The temperate japonica accessions showed 105 alleles at 50 polymorphic loci. Within three indica accessions, 266 alleles were detected at 122 polymorphic loci. Within the three tropical japonica accessions, 129 alleles were detected at 61 polymorphic loci, respectively. The average number of alleles per locus (A) 2.49, 1.2, 1.55 and 1.25 were observed in total, temperate japonica, indica, and tropical japonica accessions, respectively. Average number of alleles per polymorphic locus (Ap) 2.76, 2.1, 2.18 and 2.11 were observed in total, temperate japonica, indica, and tropical japonica accessions, respectively. The average number of gene diversity (PIC) was 0.51, indicating moderately high variation among the nine accessions. Twelve SSR markers were able to discriminate among the temperate japonica, indica, and tropical japonica rice accessions under the present study.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
审稿时长
12 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信