{"title":"大头羊adr - β3基因单链构象多态性及其与活产性状的关系","authors":"A. Ibrahim","doi":"10.21608/EJGC.2014.9922","DOIUrl":null,"url":null,"abstract":"The adrenergic receptor β3 (ADRβ3) is the major regulator of lipolysis and homeostasis and predominantly expressed in brown and white adipose tissues (Cannon and Nedergaard, 2004). The ADRβ3 gene which encodes for this receptor was studied as a candidate gene associated with growth traits, body dimensional measurements and conformation indices of Barki sheep. Single strand conformational polymorphism (SSCP) was used to identify the variation in the intron region of ADRβ3 gene for 136 male and female Barki lambs. Eighteen SSCP genotypes representing six alleles (A, B, C, D, E and H) were detected. The frequencies of these alleles were 0.331, 0.184, 0.283, 0.044, 0.073 and 0.084, respectively. The results indicated that ADRβ3 genotype significantly (P ˂ 0.05) affected marketing weight, thigh circumference, body mass index and skeletal muscle index; and also significantly (P ˂ 0.01) affected post-weaning daily gain. Least square means analysis showed that animals with genotypes AA, AC and CC had higher post-weaning daily gain, marketing weight, thigh circumference, body mass index and skeletal muscle index than animals with genotypes CD, CE and CH. In view of the obtained results, molecular marker selection using ADRβ3 gene is warranted to increase post-weaning growth rate, whole-body mass and skeletal muscle mass in Barki sheep and will be of considerable economic value to sheep producer.","PeriodicalId":31811,"journal":{"name":"Egyptian Journal of Genetics and Cytology","volume":"43 1","pages":"287-299"},"PeriodicalIF":0.0000,"publicationDate":"2014-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"SINGLE STRAND CONFORMATIONAL POLYMORPHISM OF ADRβ3 GENE AND ITS ASSOCIATION WITH LIVE PERFOR-MANCE TRAITS IN BARKI SHEEP\",\"authors\":\"A. Ibrahim\",\"doi\":\"10.21608/EJGC.2014.9922\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The adrenergic receptor β3 (ADRβ3) is the major regulator of lipolysis and homeostasis and predominantly expressed in brown and white adipose tissues (Cannon and Nedergaard, 2004). The ADRβ3 gene which encodes for this receptor was studied as a candidate gene associated with growth traits, body dimensional measurements and conformation indices of Barki sheep. Single strand conformational polymorphism (SSCP) was used to identify the variation in the intron region of ADRβ3 gene for 136 male and female Barki lambs. Eighteen SSCP genotypes representing six alleles (A, B, C, D, E and H) were detected. The frequencies of these alleles were 0.331, 0.184, 0.283, 0.044, 0.073 and 0.084, respectively. The results indicated that ADRβ3 genotype significantly (P ˂ 0.05) affected marketing weight, thigh circumference, body mass index and skeletal muscle index; and also significantly (P ˂ 0.01) affected post-weaning daily gain. Least square means analysis showed that animals with genotypes AA, AC and CC had higher post-weaning daily gain, marketing weight, thigh circumference, body mass index and skeletal muscle index than animals with genotypes CD, CE and CH. In view of the obtained results, molecular marker selection using ADRβ3 gene is warranted to increase post-weaning growth rate, whole-body mass and skeletal muscle mass in Barki sheep and will be of considerable economic value to sheep producer.\",\"PeriodicalId\":31811,\"journal\":{\"name\":\"Egyptian Journal of Genetics and Cytology\",\"volume\":\"43 1\",\"pages\":\"287-299\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2014-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Egyptian Journal of Genetics and Cytology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21608/EJGC.2014.9922\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Egyptian Journal of Genetics and Cytology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/EJGC.2014.9922","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
摘要
肾上腺素能受体β3 (ADRβ3)是脂肪分解和体内平衡的主要调节因子,主要在棕色和白色脂肪组织中表达(Cannon and Nedergaard, 2004)。研究了编码该受体的ADRβ3基因作为与巴氏羊生长性状、体尺寸和构象指标相关的候选基因。采用单链构象多态性(SSCP)对136只巴尔基公羊和母羊的ADRβ3基因内含子区变异进行了分析。共检测到18个SSCP基因型,分别代表A、B、C、D、E和H 6个等位基因。等位基因频率分别为0.331、0.184、0.283、0.044、0.073和0.084。结果表明,ADRβ3基因型对市场体重、大腿围、体重指数和骨骼肌指数有显著影响(P值小于0.05);显著影响断奶后日增重(P小于0.01)。最小二乘法分析结果表明,AA、AC和CC基因型动物断奶后日增重、市场体重、大腿围、体重指数和骨骼肌指数均高于CD、CE和CH基因型动物。由此可见,利用ADRβ3基因选择分子标记可以提高巴基羊断奶后的生长率、全身质量和骨骼肌质量,对绵羊养殖者具有重要的经济价值。
SINGLE STRAND CONFORMATIONAL POLYMORPHISM OF ADRβ3 GENE AND ITS ASSOCIATION WITH LIVE PERFOR-MANCE TRAITS IN BARKI SHEEP
The adrenergic receptor β3 (ADRβ3) is the major regulator of lipolysis and homeostasis and predominantly expressed in brown and white adipose tissues (Cannon and Nedergaard, 2004). The ADRβ3 gene which encodes for this receptor was studied as a candidate gene associated with growth traits, body dimensional measurements and conformation indices of Barki sheep. Single strand conformational polymorphism (SSCP) was used to identify the variation in the intron region of ADRβ3 gene for 136 male and female Barki lambs. Eighteen SSCP genotypes representing six alleles (A, B, C, D, E and H) were detected. The frequencies of these alleles were 0.331, 0.184, 0.283, 0.044, 0.073 and 0.084, respectively. The results indicated that ADRβ3 genotype significantly (P ˂ 0.05) affected marketing weight, thigh circumference, body mass index and skeletal muscle index; and also significantly (P ˂ 0.01) affected post-weaning daily gain. Least square means analysis showed that animals with genotypes AA, AC and CC had higher post-weaning daily gain, marketing weight, thigh circumference, body mass index and skeletal muscle index than animals with genotypes CD, CE and CH. In view of the obtained results, molecular marker selection using ADRβ3 gene is warranted to increase post-weaning growth rate, whole-body mass and skeletal muscle mass in Barki sheep and will be of considerable economic value to sheep producer.