单壁碳纳米管对人肺癌细胞的毒性:n -乙酰半胱氨酸的影响

Q4 Medicine
Nikola Jojić, V. Kojić, D. Kojić, K. Stankov, G. Bogdanovic
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引用次数: 1

摘要

背景:单壁碳纳米管(SWCNTs)已被报道在不同细胞系中诱导细胞毒性。尽管细胞毒性的机制尚不完全清楚,活性氧(ROS)的积累和氧化损伤被认为是一个可能的因素。方法:使用人肺癌细胞A549和人胎儿肺成纤维细胞MRC-5,通过MTT试验评估氧化还原状态调节剂n -乙酰半胱氨酸(NAC)存在和不存在时swcnts的细胞毒性。结果:≤250 μg/ml的swcnts诱导A594细胞的生存能力损失比MRC-5细胞大近3倍。高浓度swcnts对两种细胞系的细胞毒性相似,而单独NAC无毒。SWCNT (250 μg/ml)联合NAC对A549细胞的细胞毒性在最低NAC浓度(1.5 μg/ml)下显著降低,且与NAC单独处理在该浓度下相似。高浓度NAC与SWCNT (250 μg/ml)联合使用可增加A549和MRC-5细胞的细胞毒性。结论:A549恶性肺细胞比正常肺细胞更容易受到低浓度swcnts的影响,低浓度的n -乙酰半胱氨酸似乎具有细胞保护作用,可能是由于其抗氧化特性。[南斯拉夫]塞族共和国部长项目;173014:氧化还原信号在体内平衡中的分子机制:适应性和病理学]
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cytotoxicity of single-walled carbon nanotubes to human lung carcinoma cells: The influence of N-acetylcysteine
Background: Single-walled carbon nanotubes (SWCNTs) have been reported to induce cytotoxicity in different cell lines. Although the mechanisms underlying cytotoxicity are not fully understood, accumulation of reactive oxygen species (ROS) and oxidative damage is considered to be a likely contributing factor. Methods: Human lung carcinoma cells, A549, and human fetal lung fibroblasts, MRC-5 were used to assess the cytotoxicity of SWCNT in the presence and absence of a redox status regulator, N-acetylcysteine (NAC), via the MTT assay. Results: SWCNT induced a nearly three-fold greater loss of viability in A594 vs. MRC-5 cells at ≤250 μg/ml. SWCNT cytotoxicity at higher concentrations was similar for both cell lines, while NAC alone was non-toxic. The cytotoxicity of SWCNT (250 μg/ml) in combination with NAC to A549 cells was significantly decreased at the lowest NAC concentration (1.5 µg/ml), and was similar to NAC treatment alone at that concentration. Higher concentrations of NAC in combination with SWCNT (250 μg/ml) resulted in increased cytotoxicity in both A549 and MRC-5 cells. Conclusion: A549 malignant lung cells are more susceptible to low concentrations of SWCNT vs. normal lung cells, and low concentrations of N-acetylcysteine appear to be cytoprotective, possibly due to its antioxidant properties. [Projekat Ministarstva nauke Republike Srbije, br. 173014: Molecular mechanisms of redoxsignaling in homeostasis: Adaptation and pathology]
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来源期刊
Archive of Oncology
Archive of Oncology Medicine-Oncology
CiteScore
0.60
自引率
0.00%
发文量
5
审稿时长
12 weeks
期刊介绍: Archive of Oncology is an international oncology journal that publishes original research, editorials, review articles, case (clinical) reports, and news from oncology (medical, surgical, radiation), experimental oncology, cancer epidemiology, and prevention. Letters are also welcomed. Archive of Oncology is covered by Biomedicina Vojvodina, Biomedicina Serbica, Biomedicina Oncologica, EMBASE/Excerpta Medica, ExtraMED and SCOPUS.
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