直接超声辅助提取新鲜冬瓜叶中酚类化合物及其表征

IF 0.8 4区 工程技术 Q4 ENGINEERING, CHEMICAL
Ivana T. Karabegović, S. Stojičević, D. Veličković, N. Nikolić, M. Lazić
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引用次数: 4

摘要

研究了超声功率和超声频率对直接超声辅助提取(DUAE)鲜家居叶提取物总萃取物(TES)、总酚含量(TPC)、总黄酮含量(TFC)和抗氧化活性(AOA)的影响。以甲醇、丙酮和2-丙醇为原料,采用索氏萃取法对植物原料进行初步提取。结果表明,甲醇提取的TES产率最高(2.91±0.02),其次是丙酮和2-丙醇,每100 g新鲜植物材料的TES产率分别为2.32±0.01和2.01±0.03 g。以DUAE和甲醇为溶剂得到的鲜周叶提取物中,TPC、TFC和AOA含量分别在40.5 ~ 85.9 mg没食子酸/g干提取物、12.7 ~ 19.3 mg芦丁/g干提取物和24.6 ~ 108.2µg/ml范围内。结果表明,超声功率和提取时间的增加对提取物的TPC、TFC和AOA均有显著正相关影响(p<0.05),而超声频率的增加导致提取物的TPC、TFC和AOA降低。对粗提物进行色谱分析,鉴定出下列成分:山奈酚3-O-(6′- o -丙二醇葡萄糖苷)-7- o -葡萄糖苷、山奈酚3-O-(6′- o -丙二醇葡萄糖苷)、木犀草素5-O-(6′- o -丙二醇葡萄糖苷)-7- o -鼠李糖糖苷、木犀草素5-O-(6′- o -丙二醇葡萄糖苷)、山奈酚3-O-(6′- o -丙二醇葡萄糖苷)、山奈酚3-O-(6′- o -丙二醇葡萄糖苷)、山奈酚3-O-(6′- o -丙二醇葡萄糖苷)、山奈酚3-O-(6′- o -丙二醇葡萄糖苷、槲皮素、山奈酚4′- o -葡萄糖苷和金丝桃苷。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Direct ultrasound-assisted extraction and characterization of phenolic compounds from fresh houseleek (Sempervivum marmoreum L.) leaves
The effects of ultrasound power and frequency on the yield of total extractive substances (TES), total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity (AOA) of fresh houseleek leaves extracts obtained by direct ultrasound-assisted extraction (DUAE) were studied. Preliminary extraction of plant material was performed using methanol, acetone and 2-propanol by Soxhlet extraction. It was found that maximum TES yield could be obtained by methanol extraction (2.91±0.02), followed by acetone and 2-propanol with a TES yield of 2.32±0.01 and 2.01±0.03 g per 100 g of fresh plant material, respectively. In the fresh houseleek leaves extracts obtained by DUAE and methanol as the chosen solvent, TPC, TFC and AOA were in the ranges of: 40.5-85.9 mg gallic acid/g dry extract, 12.7-19.3 mg rutin/g dry extract and 24.6-108.2µg/ml, respectively. The results showed that the increase in the ultrasound power and extraction time have positive and significant (p<0.05) effects on the TPC, TFC and AOA, while the increase in the ultrasound frequency leads to a decrease in the TPC, TFC and AOA of the extracts. A chromatographic analysis of crude extract identified the following: Kaempferol 3-O-(6’’-O-malonylglucoside)-7-O-glucosyde, Kaempferol 3-O glucoside-7-O-rhamnoside, Luteolin 5-O-(6’’-O-malonyl­glucoside), Kaempferol 3-O-(6’’-O-acetylglucoside)-7-O-rhamnoside, Genkwanin 5-O-glu­coside, Luteolin 5-O-(6’’-O-malonylglucoside), Kaempferol 3-O-(6’’-O-malonylglucoside), Kaempferol 3-O-rhamnoside, Quercetin, Genkwanin 4’-O-glucoside and Hyperoside.
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来源期刊
Hemijska Industrija
Hemijska Industrija 工程技术-工程:化工
CiteScore
1.60
自引率
11.10%
发文量
12
审稿时长
6-12 weeks
期刊介绍: The Journal Hemijska industrija (abbreviation Hem. Ind.) is publishing papers in the field of Chemical Engineering (Transport phenomena; Process Modeling, Simulation and Optimization; Thermodynamics; Separation Processes; Reactor Engineering; Electrochemical Engineering; Petrochemical Engineering), Biochemical Engineering (Bioreactors; Protein Engineering; Kinetics of Bioprocesses), Engineering of Materials (Polymers; Metal materials; Non-metal materials; Biomaterials), Environmental Engineeringand Applied Chemistry. The journal is published bimonthly by the Association of Chemical Engineers of Serbia (a member of EFCE - European Federation of Chemical Engineering). In addition to professional articles of importance to industry, scientific research papers are published, not only from our country but from all over the world. It also contains topics such as business news, science and technology news, information on new apparatus and equipment, and articles on environmental protection.
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