小麦基因型对体外培养过量硼的响应

4区农林科学 Q3 Agricultural and Biological Sciences

Genetika-Belgrade Pub Date : 2022-01-01 DOI:10.2298/gensr2202907k

A. Kondić-Špika, S. Glogovac, D. Trkulja, A. Marjanović-Jeromela, Milica Marjanovic

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引用次数: 0

摘要

本研究的目的是利用成熟胚培养评价小麦基因型的硼耐受性。对国际小麦定位计划(ITMI)群体的79个重组自交系和3个已知耐硼(B)的塞尔维亚品种(Pobeda敏感的S、balerina耐培养基的MT和Nevesinjka耐培养基的T)进行分析，在添加15mm硼酸的改良MS培养基上进行评价。对照培养基中不含过量的b，培养1个月后测定愈伤组织鲜重(CFW)和鲜重(RFCW)的减少量。方差分析显示，基因型、培养基及其交互作用对愈伤组织生长有极显著的影响，基因型对RFCW也有显著的影响。大多数基因型(39个)对过量硼有敏感反应，23个为中等耐受性，4个为耐受性。所得结果可用于小麦育种中与耐过量B相关的qtl定位。

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Response of wheat genotypes to excess boron estimated by in vitro culture

The objective of this study was to evaluate boron tolerance of wheat genotypes using mature embryo culture. The analysis involved 79 recombinant inbred lines of the International Triticeae Mapping Initiative (ITMI) population and three Serbian varieties with known boron (B) tolerance (Pobeda - sensitive S, Balerina-medium tolerant MT, and Nevesinjka - tolerant T). The evaluation was performed on a modified MS medium to which 15 mM of boric acid was added. The control medium contained no excess B. Callus fresh weight (CFW) and reduction of fresh callus weight (RFCW) were determined after one month of cultivation. ANOVA has shown highly significant effect of genotype, the media, and their interaction to callus tissue growth and also significant genotypic effect on RFCW. Majority of genotypes (39) had sensitive reaction to excess boron, twenty-three were medium tolerant, while four of them were tolerant. The obtained results can be potentially used for mapping QTLs associated with tolerance to excess B in wheat breeding program.

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来源期刊

Genetika-Belgrade AGRONOMY-GENETICS & HEREDITY

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The GENETIKA is dedicated to genetic studies of all organisms including genetics of microorganisms, plant genetics, animal genetics, human genetics, molecular genetics, genomics, functional genomics, plant and animal breeding, population and evolutionary genetics, mutagenesis and genotoxicology and biotechnology.