绵羊血清样品中脂肪酸测定方法的验证

IF 0.5 4区化学 Q4 CHEMISTRY, MULTIDISCIPLINARY

Quimica Nova Pub Date : 2023-05-29 DOI:10.21577/0100-4042.20230009

Matheus Bazzana, Juliana Garcia, Gabriela Vilela, C. Borges, L. Faria, Nadja Alves, A. Saczk

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引用次数: 0

摘要

本研究采用低密度分散液液微萃取-酯化法测定绵羊血清样品中的亚油酸和硬脂酸。该方法旨在快速有效地提取和酯化两种脂肪酸，随后通过气相色谱/火焰电离检测进行分析。采用scheff多项式模型对三组分混合物的提取方法进行了优化。根据Eurachem Guide(2014)建立的参数对方法进行验证。LD-DLLME的最佳提取条件为:分散介质(MgCl2 0.017%) 1400µL，萃取溶剂(甲苯)400µL，分散溶剂(甲醇)1200µL。该方法具有良好的选择性、灵敏度和精密度，适用于实际样品，亚油酸和硬脂酸的平均回收率分别为98.54%和103.83%。LD-DLLME方法优于传统的反刍动物血清中脂肪酸的分析方法。实际样品分析表明，该方法对反刍动物体内这些物质的监测是有效的。

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VALIDATION OF AN ANALYTICAL METHOD FOR THE DETERMINATION OF FATTY ACIDS IN SHEEP BLOOD SERUM SAMPLES

Low-density dispersive liquid-liquid microextraction with a subsequent esterification step was proposed in this study for the determination of linoleic acid and stearic acid in sheep blood serum samples. The method developed aimed to quickly and efficiently extract and esterify both fatty acids with subsequent analysis by gas chromatography/flame ionization detection. The extraction method was optimized with Scheffé’s polynomial model for three-component mixtures. The method was validated according to the parameters established by Eurachem Guide (2014). The optimal extraction conditions for LD-DLLME were 1400 µL of dispersion medium (MgCl2 0.017%), 400 µL of extractor solvent (toluene) and 1200 µL of dispersion solvent (methanol). The method performance showed adequate selectivity, sensitivity and precision to be applied to real samples, with an average recovery of 98.54% for linoleic acid and 103.83% for stearic acid. LD-DLLME was superior to the traditional method of analysis, which has been used until now for the determination of fatty acids in blood serum samples from ruminants. The analysis of real samples showed that the developed method is efficient for monitoring these substances in ruminants.

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来源期刊

Quimica Nova 化学-化学综合

CiteScore

1.60

自引率

12.50%

发文量

审稿时长

2-4 weeks

期刊介绍： Química Nova publishes in portuguese, spanish and english, original research articles, revisions, technical notes and articles about education in chemistry. All the manuscripts submitted to QN are evaluated by, at least, two reviewers (from Brazil and abroad) of recognized expertise in the field of chemistry involved in the manuscript. The Editorial Council can be eventually asked to review manuscripts. Editors are responsible for the final edition of QN.