禽分枝杆菌亚种。副结核降低铁诱导巨噬细胞中铁portin 1 mrna表达的调控

IF 0.2 4区 农林科学 Q2 Veterinary
B. L. Sánchez, José Ángel Gutiérrez Pabello, Gerardo Enrique Medina Basulto, T. R. Evangelista, E. D. Aparicio, S. Oshima
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引用次数: 0

摘要

引用本文为:Landeros Sanchez B, Gutierrez Pabello JA, Medina Basulto GE, Renteria Evangelista TB, Diaz Aparicio E, Oshima S.禽分枝杆菌亚种。副结核病下调铁诱导巨噬细胞铁转运蛋白1的mRNA表达。墨西哥兽医局。2016; 3(1)。禽分枝杆菌亚种。副结核(MAP)是约翰氏病的病原体。MAP能够适应先天宿主反应的机制尚不清楚。我们通过实时PCR检测了在鸟分枝杆菌亚群中孵育的小鼠巨噬细胞系J774中铁转运蛋白1 (FPN1) mRNA的表达水平。副结核(MAP)或MAP粗蛋白提取物。活MAP感染以感染多重性(MOI)依赖的方式降低FPN1 mRNA水平。MOIs比例为20:1和15:1时,巨噬细胞的FPN1基因表达没有变化,而MOIs比例为10:1和5:1时,巨噬细胞的FPN1基因表达分别下降了50%和80%。50、100、150和200µg/mL MAP粗提物(ATCC19698)处理巨噬细胞使FPN1 mRNA表达降低25%。此外,400µM硝酸三乙酸铁(FeNTA)铁超载处理后FPN1 mRNA的上调被活MAP (MOI 20:1)消除了约70%。我们的数据揭示了MAP对FPN1 mRNA的抑制作用,并提出了一种可能在宿主铁调节中发挥作用的细菌机制。图1所示。鸟分枝杆菌亚种感染。副结核(MAP)下调巨噬细胞fpn1基因表达。MAP以不同感染倍数(MOIs)感染小鼠巨噬细胞J774 6小时。提取总RNA,实时荧光定量PCR检测基因表达。将结果归一化为GAPDH,并以相对表达(URE)为单位进行表达。结果为3个独立实验的平均值±标准差,每个实验3个重复。差异有统计学意义,P = 0.05。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
El Mycobacterium avium subesp. paratuberculosis disminuye la regulación de la expresión del ARNm de la ferroportina 1 en los macrófagos inducidos con hierro
Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Landeros Sanchez B, Gutierrez Pabello JA, Medina Basulto GE, Renteria Evangelista TB, Diaz Aparicio E, Oshima S. Mycobacterium avium subsp. paratuberculosis down-regulates mRNA expression of iron-induced macrophage Ferroportin 1. Veterinaria Mexico OA. 2016;3(1). doi: 10.21753/vmoa.3.1.361 Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne’s disease. The mechanisms by which MAP is able to adapt to the innate host response are still unclear. We examined Ferroportin 1 (FPN1) mRNA expression levels via real-time PCR of the mouse macrophage cell line J774 that was incubated in the presence of Mycobacterium avium subsp. paratuberculosis (MAP) or MAP crude protein extract. Infection with live MAP decreased FPN1 mRNA levels in a multiplicity of infection (MOI)-dependent fashion. Macrophages infected with MOIs of 20:1 and 15:1 did not show any change in FPN1 gene expression, whereas MOIs of 10:1 and 5:1 induced a decrease of 50 and 80%, respectively. Macrophages treated with 50, 100, 150 and 200 µg/mL of MAP crude extract (ATCC19698) decreased FPN1 mRNA expression by 25%. Additionally, up-regulation of FPN1 mRNA by an iron overload treatment of 400 µM of ferric nitrilotriacetate (FeNTA) was abrogated by live MAP (MOI 20:1) by approximately 70%. Our data revealed an inhibitory effect of MAP on FPN1 mRNA and suggested a bacterial mechanism that may play a role in host iron regulation. Figure 1. Infection by Mycobacterium avium subsp. paratuberculosis (MAP) down-regulates macrophage fpn1 gene expression. Murine macrophages, J774, were infected with MAP at different multiplicities of infection (MOIs) for 6 hours. Total RNA was extracted and gene expression was measured by real-time PCR. Results were normalized to GAPDH and expressed as units of relative expression (URE). Results are the mean ± standard deviation of 3 independent experiments with 3 replicates each. Statistical significance, P = 0.05.
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来源期刊
Veterinaria Mexico
Veterinaria Mexico VETERINARY SCIENCES-
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审稿时长
>12 weeks
期刊介绍: Veterinaria México OA (ISSN 2448-6760) is an online scientific journal edited by Universidad Nacional Autónoma de México (UNAM). The journal is Open Access and follows UNAM''s initiative, to transmit knowledge free of charge to the readership and authors, with no Article Processing Charges. This journal publishes advances in Veterinary Sciences and Animal Production, and to reach more lectures across the world the journal was updated since 2014 from its predecessor printed in paper Veterinaria México (ISSN 0301-5092) and its digital version (ISSN 2007-5472).
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