Somayeh Heidarizadi, N. Abbasi, K. Asadollahi, S. Rezaei, A. Moayeri, M. Azizi
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In three groups of Control, vehicle and Treatment, after laminectomy, spinal cord contusion model was performed using the Weight drop technique. Immediately after the injury, 10μl Dulbecco's Modified Eagle Medium (DMEM) alone or with 10 cells were injected to vehicle and Treatment Group animals. For culturing cells, the olfactory mucosa of 7-day-old male Wistar rats was used. Motor function of animals in all groups, was evaluated in the first 48 hours daily and then weekly for eight weeks. Results: Comparing the results of the second to eighth week of the study showed significant differences in the group receiving the OECs with the control group (P<0.05). Conclusion: The results indicate a positive influence of the olfactory ensheathing cells in functional improvement of spinal cord injury in the acute phase of injury.","PeriodicalId":15047,"journal":{"name":"Journal of Basic Research in Medical Sciences","volume":"4 1","pages":"30-36"},"PeriodicalIF":0.0000,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Effect of olfactory ensheathing cells (OECs) transplantation on functional recovery in acute phase of spinal contused rats\",\"authors\":\"Somayeh Heidarizadi, N. Abbasi, K. Asadollahi, S. Rezaei, A. Moayeri, M. Azizi\",\"doi\":\"10.18869/ACADPUB.JBRMS.4.1.30\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Introduction: Spinal cord injuries (SCI) lead to permanent irreversible functional deficits. Poor prognosis of patients is the motivation of searching a treatment for the chronic injury. 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Motor function of animals in all groups, was evaluated in the first 48 hours daily and then weekly for eight weeks. Results: Comparing the results of the second to eighth week of the study showed significant differences in the group receiving the OECs with the control group (P<0.05). 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引用次数: 3
摘要
简介:脊髓损伤(SCI)导致永久性不可逆的功能缺陷。患者预后不良是寻求治疗慢性损伤的动力。植入干细胞为我们提供了一个很有前途的策略。同时,嗅鞘细胞(OECs)的应用也取得了很好的效果。本研究旨在评价oec移植对急性脊髓损伤大鼠功能恢复的影响。材料和方法:本研究选用18只体重约210±10 gr的成年雄性Wistar大鼠进行脊髓损伤研究。随机分为4组:假手术组(n=3)、对照组(n=6)、实验组(n=3)和实验组(n=6)。假手术组仅在T9脊髓区行椎板切除术。对照组、对照组和实验组三组,椎板切除术后采用重量下降技术建立脊髓挫伤模型。损伤后立即注射10μl Dulbecco's Modified Eagle Medium (DMEM)给对照组和治疗组动物。细胞培养采用7日龄雄性Wistar大鼠嗅粘膜。各组动物的运动功能在前48小时每天进行评估,然后每周进行评估,持续8周。结果:研究第2周至第8周,OECs组与对照组比较,差异有统计学意义(P<0.05)。结论:嗅鞘细胞对脊髓损伤急性期功能改善有积极作用。
Effect of olfactory ensheathing cells (OECs) transplantation on functional recovery in acute phase of spinal contused rats
Introduction: Spinal cord injuries (SCI) lead to permanent irreversible functional deficits. Poor prognosis of patients is the motivation of searching a treatment for the chronic injury. Planting stem cells provides us with a promising strategy. In the meanwhile, the use of olfactory ensheathing cells (OECs) has shown very good results. This study aims at evaluating the effect of transplanted OECs on functional recovery of acute SCI in rats. Materials and methods: In this study, eighteen adult male Wistar rats weighting approximately 210 ± 10 gr were used to study spinal cord injury. They were randomly divided into four groups: Sham (n = 3), Control (n = 6), Vehicle (n=3) and Treatment (n=6). In the Sham Group, only laminectomy was performed in the area of T9 spinal cord. In three groups of Control, vehicle and Treatment, after laminectomy, spinal cord contusion model was performed using the Weight drop technique. Immediately after the injury, 10μl Dulbecco's Modified Eagle Medium (DMEM) alone or with 10 cells were injected to vehicle and Treatment Group animals. For culturing cells, the olfactory mucosa of 7-day-old male Wistar rats was used. Motor function of animals in all groups, was evaluated in the first 48 hours daily and then weekly for eight weeks. Results: Comparing the results of the second to eighth week of the study showed significant differences in the group receiving the OECs with the control group (P<0.05). Conclusion: The results indicate a positive influence of the olfactory ensheathing cells in functional improvement of spinal cord injury in the acute phase of injury.