伊拉姆市医院金黄色葡萄球菌临床分离株对万古霉素和甲氧西林的耐药性测定

A. Rostamzad, Nabi Rostamneia, F. Pourahmad, M. Shamsi
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引用次数: 2

摘要

本研究采用表型和基因型方法,对伊朗伊拉姆两所政府医院患者分离的金黄色葡萄球菌(S. aureus)菌株进行了oxacillin敏感性检测。材料与方法:从不同临床标本中分离出200株金黄色葡萄球菌,包括血液(31%)、伤口(20%)、尿液(21%)、导管(7%)、痰液(12%)和其他(9%)。采用圆盘扩散法对耐甲氧西林金黄色葡萄球菌分离株进行检测,并用MuellerHinton琼脂培养基对oxacillin (1μg)和头孢西丁(30μg)进行PCR检测。此外,还检测了分离株的抗生素谱。结果:本研究纳入的200株金黄色葡萄球菌中,35.96%为MRSA。盘片扩散法耐药率分别为:青霉素85.96%、万古霉素0%、氨苄西林87.71%、庆大霉素48.25%、红霉素54.25%、克林霉素32.45%、阿米卡星21.05%、环丙沙星42.10%、四环素51.75%、复方新诺明42.10%。膜片扩散法检测MRSA的敏感性和特异性分别为33.33%和35.96%。经PCR检测,MRSA分离株中MecA和vanA基因的检出率分别为35.96%和0%。奥西林和头孢西丁纸片扩散法的灵敏度分别为92.68%和100%,特异度为98.8%。结论:头孢西丁纸片扩散法优于与PCR检测MecA基因结果相似的奥西林纸片扩散法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Determination of vancomycin and methicillin resistance in clinical isolates of Staphylococcus aureus in hospitals of Ilam city
Introduction: In this study, using the phenotypic and genotypic methods, oxacillin susceptibility in Staphylococcus aureus (S. aureus) strains isolated from patients at two government hospitals in Ilam, Iran was tested. Materials and methods: Out of 200 S. aureus isolates from different human clinical specimens consisting of blood (31%), wound (20%), urine (21%), catheters (7%), sputum (12%), others (9%) were collected. The methicillin resistant S. aureus isolates were investigated using disk diffusion methods and oxacillin (1μg) and cefoxitin (30μg), on MuellerHinton agar were used, and MecA and vanA genes were detected by PCR. In addition, the isolates were tested for their antibiogram profiles. Results: Among 200 S. aureus strains included in this study, 35.96% were MRSA. The percentage of resistance by disk diffusion method was as below: penicillin 85.96%, vancomycin 0%, ampicillin 87.71%, gentamicin 48.25% erythromycin 54.25%, clindamycin 32.45%, amikacin 21.05%, ciprofloxacin 42.10%, tetracycline 51.75% and co-trimoxazole 42.10%. Phenotyping method by disk diffusion method using oxacillin and cefoxitin for detecting of MRSA showed sensitivity and specificity of about 33.33% and 35.96%, respectively. Presence of MecA and vanA genes in MRSA isolates by PCR were 35.96% and 0%, respectively. The oxacillin and cefoxitin disk diffusion methods showed 92.68% and 100% sensitivity, respectively, and 98.8% specificity. Conclusion: Our finding showed that, the cefoxitin disk diffusion method is better in compared to the oxacillin disk diffusion similar to results from detecting of MecA gene in PCR as a golden test.
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