中国黄鹌鹑羽毛中MC1R基因多态性的鉴定及其与黑色素含量的关系

IF 1.1 4区农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Brazilian Journal of Poultry Science Pub Date : 2023-04-07 DOI:10.1590/1806-9061-2022-1648

Y. Qi, X. Zhang, Y. Pang, B. Yuan, J. Cheng

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引用次数: 0

摘要

MC1R在控制黑色素细胞中合成的黑色素类型中起着至关重要的作用，而黑色素细胞对鸟类的羽毛颜色有很大的影响。在MC1R基因编码区发现1个g.16796362G/T SNP，导致氨基酸序列发生Met120Ile突变。Met120Ile突变位于MC1R蛋白的第三跨膜结构域，降低了蛋白质的稳定性。g.16796362G/T基因座具有中等多态性，与羽毛黑色素含量显著相关。AA基因型皮肤组织中总黑色素和泛黑素含量显著低于AB和BB基因型，而不同基因型羽毛中MC1R mRNA的表达量无显著差异。本实验表明，Met120Ile突变可影响中国黄鹌鹑MC1R蛋白的功能，改变黑素的生物合成。

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Identification of Polymorphism in the MC1R Gene and Its Association with the Melanin Content in Feathers of Chinese Yellow Quails

MC1R plays a crucial role in controlling the type of melanin synthesized in the melanocytes, which greatly affects plumage color in birds. One g.16796362G/T SNP was found in the MC1R gene coding region, which caused a Met120Ile mutation in the amino acid sequence. The Met120Ile mutation was located in the third transmembrane domain of the MC1R protein and decreased protein stability. The g.16796362G/T locus achieved medium polymorphism and had significant association with feather melanin content in Chinese yellow quails. The contents of total melanin and pheomelanin with AA genotype were significant lower than those with AB or BB genotypes in skin tissues, while the expression levels of MC1R mRNA had no significant difference in feathers with different genotypes. This experiment indicated that the Met120Ile mutation could affect the function of the MC1R protein and change the biosynthesis of melanin in Chinese yellow quails.

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来源期刊

Brazilian Journal of Poultry Science 农林科学-奶制品与动物科学

CiteScore

1.80

自引率

9.10%

发文量

审稿时长

>12 weeks

期刊介绍： A Revista Brasileira de Ciência Avícola surgiu em 1999 a partir da necessidade que a comunidade científica possuía de um periódico para veiculação e publicação de seus trabalhos, com a publicação de três números anuais. A Revista conta hoje com um corpo editorial altamente qualificado e com artigos científicos desenvolvidos pelos maiores especialistas da área, o que a cada dia atrai mais leitores em busca de inovação e respaldo técnico. Devido à credibilidade que conquistou pelos esforços de sus autores, relatores e revisores, a Revista ganhou caráter de coleção, sendo consultada como fonte segura de estudo desenvolvidos na Avicultura. A partir de 2003 – volume 5 -, a Revista passou a chamar-se Brazilian Journal of Poultry Science, e todos os trabalhos passaram a ser publicados em inglês. No mesmo ano subiu para quatro o número de revistas por volume, ampliando-se assim os trabalhos publicados anualmente.