病毒体高通量测序分析揭示了葡萄植株的多重感染

IF 0.8 4区农林科学 Q3 AGRONOMY

Ciencia Rural Pub Date : 2023-03-13 DOI:10.1590/0103-8478cr20220284

T. Fajardo, A. Nhani Junior, O. Nickel

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引用次数: 0

摘要

摘要:在葡萄的植物检疫问题中，病毒以其降低葡萄品质和产量的能力而引人注目。然而，检测和识别葡萄藤中的病毒感染可能具有挑战性。这项研究进行了高通量测序(HTS)的病毒病原体存在于藤显示病毒样症状，以阐明病因。HTS分析报告，在一株叶片边缘轻微卷曲的杂交葡萄藤中，存在四种病毒和类病毒，可能与观察到的症状有关。确定的完整基因组与先前鉴定的同源病原体分离株具有高度的遗传同一性。

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Analysis of virome by high-throughput sequencing disclosed multiple infection in a single grapevine plant

ABSTRACT: Among phytosanitary problems of the grapevine, viruses stand out for their capacity of reducing the quality and yield of grapes. However, detecting and identifying viral infections in grapevines can be challenging. This study performed a high throughput sequencing (HTS) of the viral pathogens present in a vine showing virus-like symptoms to elucidate the etiology. HTS analysis reported in a hybrid grapevine with mild curling down of leaf edges, the presence of four viruses and viroids, which were probably implicated in the observed symptoms. The determined complete genomes showed high genetic identities with previously characterized isolates of homologous pathogens.

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来源期刊

Ciencia Rural AGRONOMY-

CiteScore

1.70

自引率

0.00%

发文量

233

审稿时长

2-4 weeks

期刊介绍： The purpose of Ciência Rural is to publish the results of original research, note and reviews which contribute significantly to knowledge in Agricultural Sciences. Preference will be given to original articles that develop news concepts or experimental approaches and are not merely repositories of scientific data. The decison of acceptance for publication lies with the Editors and is based on the recommendations of Editorial Comission, Area Committee and/ or ad hoc reviewers. The editors and reviewers are external to the institution.