水稻核rbcL基因过表达诱导的抗旱性

IF 0.7 4区 农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY
João Augusto Vieira de Oliveira, R. P. Vianello, A. C. Lanna, B. Dedicova, Dhiôvanna Corrêia Rocha, C. Brondani
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引用次数: 0

摘要

摘要本研究旨在研究Rubisco核大亚基(rbcL)的过表达是否能提高转基因水稻(GM) BRSMG的抗旱性。对同一品种转基因和非转基因植株在丰水和亏水两种水分处理下的生长情况进行了比较。在两种处理下,转基因植株的农艺性状(包括产量)均优于非转基因植株。通过定量聚合酶链反应,转基因植株在WW和WD中rbcL基因的表达量显著增加,脱落酸的表达量也显著增加。通过RNAseq分析,在水分限制后的第2阶段,转基因植物中发现的上调基因几乎增加了三倍,表明对水分不足有更大的保护。过表达rbcL基因的转基因水稻抗旱性提高可能与细胞代谢保护相关基因的高表达和一系列生理变化有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Drought tolerance induced by the overexpression of the nuclear rbcL gene in rice
Abstract The objective of this work was to determine whether the overexpression of the nuclear Rubisco large subunit (rbcL) improves the drought tolerance of the genetically modified (GM) BRSMG Curinga upland rice (Oryza sativa) cultivar. GM and non-genetically modified (NGM) plants of the same cultivar were compared under the two following water treatments: well watered (WW) and water deficit (WD). The performance of the agronomic traits of GM plants, including grain yield, was superior to that of NGM plants in both treatments. By quantitative polymerase chain reaction, GM plants show a significantly higher expression of the rbcL gene in both WW and WD, as well as a larger amount of abscisic acid. With the RNAseq analysis, almost three times more upregulated genes are identified in GM plants in stage 2 after water restriction, indicating a greater protection against water deficit. The higher expression of genes related to the protection of the cellular metabolism and a series of physiological alterations may be involved in the increase in the drought tolerance of GM rice plants overexpressing the rbcL gene.
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来源期刊
Pesquisa Agropecuaria Brasileira
Pesquisa Agropecuaria Brasileira 农林科学-农业综合
CiteScore
1.20
自引率
0.00%
发文量
45
审稿时长
9-18 weeks
期刊介绍: Pesquisa Agropecuária Brasileira – PAB – is issued monthly by Empresa Brasileira de Pesquisa Agropecuária – EMBRAPA, affiliated to Ministry of Agriculture, Livestock and Food Supply. PAB publishes original scientific-technological articles on Plant Physiology, Plant Pathology, Crop Science, Genetics, Soil Science, Food Technology and Animal Science. Its abbreviated title is Pesq. agropec. bras., and it should be used in bibliographies, footnotes, references and bibliographic strips.
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