Ricardo Pereira Pinto, F. Mata, P. Pires, M. Barros, J. Araújo, M. Vaz-Velho
{"title":"采用高效液相色谱法对猪饲粮中添加甜菜粕防治粪臭菌进行了定量分析","authors":"Ricardo Pereira Pinto, F. Mata, P. Pires, M. Barros, J. Araújo, M. Vaz-Velho","doi":"10.1590/1678-992x-2022-0093","DOIUrl":null,"url":null,"abstract":": This study aimed to determine the efficacy of a finishing diet added with sugar beet pulp to reduce backfat skatole of entire male pigs, using the optimised high-performance liquid chromatographic (HPLC) method. The study comprised 72 males Pietrain (Large White × Landrace), divided into two groups of 36 animals each. Pigs in group A (treatment) were fed a supplemented formula (addition of 10 % beet pulp). while animals in group B (control) received a commercial feed, both for a period of 14 days before slaughter. The isocratic HPLC method achieved the chromatographic separation of indolic compounds in approximately 3 min. Skatole was significantly lower ( p = 0.002) in group A, showing that beet root supplementation reduced skatole levels in pig fat. In addition, the optimised HPLC method was reliable, less time-consuming, and showed a resolution suitable for small amounts of skatole.","PeriodicalId":49559,"journal":{"name":"Scientia Agricola","volume":"154 1","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2023-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"The use of sugar beet pulp in pig diet to control skatole analysed by HPLC quantification method\",\"authors\":\"Ricardo Pereira Pinto, F. Mata, P. Pires, M. Barros, J. Araújo, M. Vaz-Velho\",\"doi\":\"10.1590/1678-992x-2022-0093\",\"DOIUrl\":null,\"url\":null,\"abstract\":\": This study aimed to determine the efficacy of a finishing diet added with sugar beet pulp to reduce backfat skatole of entire male pigs, using the optimised high-performance liquid chromatographic (HPLC) method. The study comprised 72 males Pietrain (Large White × Landrace), divided into two groups of 36 animals each. Pigs in group A (treatment) were fed a supplemented formula (addition of 10 % beet pulp). while animals in group B (control) received a commercial feed, both for a period of 14 days before slaughter. The isocratic HPLC method achieved the chromatographic separation of indolic compounds in approximately 3 min. Skatole was significantly lower ( p = 0.002) in group A, showing that beet root supplementation reduced skatole levels in pig fat. In addition, the optimised HPLC method was reliable, less time-consuming, and showed a resolution suitable for small amounts of skatole.\",\"PeriodicalId\":49559,\"journal\":{\"name\":\"Scientia Agricola\",\"volume\":\"154 1\",\"pages\":\"\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2023-05-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Scientia Agricola\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1590/1678-992x-2022-0093\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Scientia Agricola","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1590/1678-992x-2022-0093","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
The use of sugar beet pulp in pig diet to control skatole analysed by HPLC quantification method
: This study aimed to determine the efficacy of a finishing diet added with sugar beet pulp to reduce backfat skatole of entire male pigs, using the optimised high-performance liquid chromatographic (HPLC) method. The study comprised 72 males Pietrain (Large White × Landrace), divided into two groups of 36 animals each. Pigs in group A (treatment) were fed a supplemented formula (addition of 10 % beet pulp). while animals in group B (control) received a commercial feed, both for a period of 14 days before slaughter. The isocratic HPLC method achieved the chromatographic separation of indolic compounds in approximately 3 min. Skatole was significantly lower ( p = 0.002) in group A, showing that beet root supplementation reduced skatole levels in pig fat. In addition, the optimised HPLC method was reliable, less time-consuming, and showed a resolution suitable for small amounts of skatole.
期刊介绍:
Scientia Agricola is a journal of the University of São Paulo edited at the Luiz de Queiroz campus in Piracicaba, a city in São Paulo state, southeastern Brazil. Scientia Agricola publishes original articles which contribute to the advancement of the agricultural, environmental and biological sciences.